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291 results for Protein Purification

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Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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CM Sephadex™ C-25 Ion Exchange Chromatography Media

CM Sephadex™ C-25 Ion Exchange Chromatography Media

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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Affinity coupling media, CNBr-activated Sepharose™ 4

Affinity coupling media, CNBr-activated Sepharose™ 4

Supplier: Cytiva

CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.

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Reversed phase chromatography media, SOURCE™ 15RPC

Reversed phase chromatography media, SOURCE™ 15RPC

Supplier: Cytiva

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.

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Gel filtration media, Sephadex™ G-100

Gel filtration media, Sephadex™ G-100

Supplier: Cytiva

Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.

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Ion exchange chromatography media, CM Sepharose™ Fast Flow

Ion exchange chromatography media, CM Sepharose™ Fast Flow

Supplier: Cytiva

CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Sepharose™ CL-6B Gel Filtration Base Matrix

Sepharose™ CL-6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow

Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow

Supplier: Cytiva

Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.

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Affinity chromatography media, MabSelect SuRe™

Affinity chromatography media, MabSelect SuRe™

Supplier: Cytiva

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

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Ion exchange chromatography media, DEAE Sephacel™

Ion exchange chromatography media, DEAE Sephacel™

Supplier: Cytiva

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

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Protein A from Staphylococcus aureus, MP Biomedicals

Supplier: MP Biomedicals

Protein A is a recombinant Fc-binding protein from Staphylococcus aureus, Cowan strain-1 gene. The product is a carboxy truncated material containing 301 amino acid residues. The non-binding regions have been removed to minimise potenial steric interference.

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Affinity coupling media, Epoxy-Activated Sepharose™ 6B

Supplier: Cytiva

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

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Concentrator

Supplier: G-Biosciences

The concentrator is a high molecular weight polymer which will not migrate across the dialysis membrane. The concentrator is placed against the dialysis membrane of the Tube-O-DIALYZER™, where it rapidly absorbs water from the sample and reduces the sample volume. The concentrator can be used with dialysis bags and other dialysis devices.

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Biacore consumables and accessories

Biacore consumables and accessories

Supplier: Cytiva

Biacore maintenance kits are convenient kits for proper instrument maintenance.

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Chelex® 100, sodium form

Supplier: Merck

Chelex® 100 Chelating Resin is a weak cation exchange resin that has high selectivity, approximately 5,000:1, for copper, iron, and other heavy metal ions over monovalent cations. Chelex 100 Resin is made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

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Ion exchange chromatography media, SP Sepharose® fast flow

Supplier: Merck

Sepharose Fast Flow is composed of macroporous, beaded, cross-linked agarose and modified with sulphopropyl (SP) strong cation exchange groups which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.

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SMART Digest™ ImmunoAffinity (IA) kits

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Kits are designed to provide fast, simple, highly reproducible and sensitive immunocapture and protein digestion in a single well. These kits remove the challenges often associated with the process of biomarker quantitation, by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight.

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SMART Digest™ Chymotrypsin Kits

Supplier: Thermo Fisher Scientific

SMART Digest™ Chymotrypsin Kits are designed to provide a complimentary digestion option to the SMART Digest™ Trypsin Kits which incorporate trypsin functionality. The Chymotrypsin enzyme functionality allows digestion of proteins at different points of the amino acid chain compared to trypsin. This allows for different/additional structural information to be obtained when trying to characterise a protein. This option can also be used if a protein does not contain the specific point where trypsin cuts the protein chain.

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Protein digestion kits, trypsin kits, SMART Digest™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ Trypsin Kits are designed to provide a simple to use, fast, highly reproducible and robust process for digestion of proteins in a high throughput format, meeting the needs of biopharmaceutical, bioanalytical and QA/QC environments.

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SMART Digest™ proteinase K kits

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ Proteinase K Kits are designed to provide a simple to use, fast, highly reproducible and robust process for digestion of proteins in a high throughput format, meeting the needs of biopharmaceutical, bioanalytical and QA/QC environments. This version of the kit utilises Proteinase K, which cleaves the peptide bond adjacent to the carboxyl gropu of aliphatic and aromatic amino acids with blocked alpha amino groups.

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Human IgA purification kit, LigaTrap®

Supplier: LIGATRAP TECHNOLOGIES

LigaTrap® Human IgA Purification Kit with LT technology utilises novel, patented ligands, designed for IgA antibody purification. Kit provides all the necessary reagents for fast, convenient micro-scale purification of human IgA in just ten easy steps.

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Mouse IgM purification kit

Supplier: LIGATRAP TECHNOLOGIES

LigaTrap® Mouse IgM Purification Kit with LT technology utilises novel, patented ligands, designed for IgM antibody purification. Kit provides all the necessary reagents for fast, convenient micro-scale purification of mouse IgM in just ten easy steps.

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