You searched for: Protein Purification

Supplier: Cytiva

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

Supplier: Cytiva

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

Supplier: Cytiva

Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

Supplier: Cytiva

Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Supplier: Cytiva

SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.

Supplier: Cytiva

SP Sephadex™ C-25 is a strong cation exchanger. The ion exchange group is a sulphopropyl group which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.

Supplier: Cytiva

Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

Supplier: Cytiva

Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.

Supplier: Cytiva

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

Supplier: Cytiva

Octyl Sepharose™ 4 Fast Flow resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilised to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

Supplier: Cytiva

PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography medium with a selectivity that allows generic purification of supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.

Supplier: Cytiva

CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

Supplier: Cytiva

Capto™ S ImpAct is a strong cation exchange chromatography medium (resin) developed for polishing of monoclonal antibodies (MAbs) and other biomolecules.

Supplier: Cytiva

SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.

Supplier: Cytiva

Mono-sized 15 um rigid beads gives low back pressure and high flow rates allowing for high productivity.

Supplier: Cytiva

The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

Supplier: Cytiva

MabSelect SuRe™ LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes.

Supplier: Cytiva

DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.

Supplier: Cytiva

Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Supplier: Cytiva

Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

Supplier: Cytiva

Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

Supplier: Cytiva

Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.

Supplier: Cytiva

MabSelect™ is a protein A affinity resin designed for capturing monoclonal antibodies (mAbs) from large sample volumes.

Supplier: Cytiva

Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.

Supplier: Cytiva

Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.

Supplier: Cytiva

Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Supplier: Cytiva

CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.

Supplier: Cytiva

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.

Supplier: Cytiva

Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.