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You searched for: Protein Purification

Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow

Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow

Supplier: Cytiva

IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.

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Ion exchange chromatography media, ANX Sepharose™ 4 Fast Flow (High Sub)

Ion exchange chromatography media, ANX Sepharose™ 4 Fast Flow (High Sub)

Supplier: Cytiva

ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.

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Ion exchange chromatography media, Q Sepharose™ High Performance

Ion exchange chromatography media, Q Sepharose™ High Performance

Supplier: Cytiva

Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.

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Multimodal chromatography media, Capto™ MMC

Multimodal chromatography media, Capto™ MMC

Supplier: Cytiva

Capto™ MMC is a multimodal cation exchanger with a different selectivity, compared with traditional ion exchangers.

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Gel filtration media, Sephacryl™ High Resolution

Gel filtration media, Sephacryl™ High Resolution

Supplier: Cytiva

Sephacryl™ High Resolution (HR) gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

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Gelatin-Sepharose® 4B

Gelatin-Sepharose® 4B

Supplier: Cytiva

Gelatin Sepharose® 4B affinity media with immobilised gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose® 4B is bovine gelatin coupled to Sepharose® 4B by the cyanogen bromide method.

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Accessories and spare parts for ÄKTApilot

Accessories and spare parts for ÄKTApilot

Supplier: Cytiva

TC gasket, 25/4

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Affinity chromatography media, Streptavidin Sepharose™ High Performance

Affinity chromatography media, Streptavidin Sepharose™ High Performance

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.

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CM Sephadex™ C-50 Ion Exchange Chromatography Media, Cytiva

CM Sephadex™ C-50 Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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QAE Sephadex™ Ion Exchange Chromatography Media, Cytiva

QAE Sephadex™ Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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Affinity coupling media, CNBr-Activated Sepharose™ 4B

Affinity coupling media, CNBr-Activated Sepharose™ 4B

Supplier: Cytiva

CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

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Q Sepharose™ XL Ion Exchange Chromatography Media, Cytiva

Q Sepharose™ XL Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow media.

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Affinity chromatography media, MabSelect™ PrismA

Affinity chromatography media, MabSelect™ PrismA

Supplier: Cytiva

MabSelect™ PrismA has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing future demands in mAb processing to be met.

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Sepharose™ CL-4B Octyl Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

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Gel filtration media, Sephadex™ G-15

Gel filtration media, Sephadex™ G-15

Supplier: Cytiva

Sephadex™ G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.

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Affinity chromatography media, IgG Sepharose™ 6 Fast Flow

Affinity chromatography media, IgG Sepharose™ 6 Fast Flow

Supplier: Cytiva

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it.

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Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media

Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media

Supplier: Cytiva

Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.

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DEAE Sephadex™ A-25 Ion Exchange Chromatography Media, Cytiva

DEAE Sephadex™ A-25 Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

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Ion exchange chromatography media, SOURCE™ 15S

Ion exchange chromatography media, SOURCE™ 15S

Supplier: Cytiva

SOURCE™ 15S is a synthetic high performance, preparative, chromatography medium, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

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DEAE Sephadex™ A-50 Ion Exchange Chromatography Media, Cytiva

DEAE Sephadex™ A-50 Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

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Gel filtration media, Sephadex™ G-75

Gel filtration media, Sephadex™ G-75

Supplier: Cytiva

Sephadex™ G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

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Gel filtration calibration kits

Gel filtration calibration kits

Supplier: Cytiva

Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns, such as Superdex, Superose and Sephacryl.

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Ion exchange chromatography media, SOURCE™ 30S

Ion exchange chromatography media, SOURCE™ 30S

Supplier: Cytiva

SOURCE 30S is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix It is modified with sulphonate (S) strong cation exchange groups.

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Affinity chromatography media, MabSelect Xtra™

Affinity chromatography media, MabSelect Xtra™

Supplier: Cytiva

MabSelect Xtra has the same recombinant Protein A ligand as MabSelect but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect.

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Ion exchange chromatography media, SP Sepharose™ High Performance

Ion exchange chromatography media, SP Sepharose™ High Performance

Supplier: Cytiva

SP Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.

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Butyl-S Sepharose™ 6 Fast Flow Hydrophobic interaction chromatography media

Butyl-S Sepharose™ 6 Fast Flow Hydrophobic interaction chromatography media

Supplier: Cytiva

Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.

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Sepharose™ CL-2B Gel Filtration Base Matrix

Sepharose™ CL-2B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Immobilised metal ion affinity chromatography (IMAC) media, Ni Sepharose™ excel

Immobilised metal ion affinity chromatography (IMAC) media, Ni Sepharose™ excel

Supplier: Cytiva

Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

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Superose™ 12 Gel Filtration Media, Prep Grade

Superose™ 12 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

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Ion exchange chromatography media, IEX Sepharose™ Fast Flow

Ion exchange chromatography media, IEX Sepharose™ Fast Flow

Supplier: Cytiva

Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades.

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