You searched for: Cellular Assays
Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
TUNEL assay kits
Supplier: Biotium
TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.
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Cell proliferation assay kit, WST-1
Supplier: Cayman Chemical
WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.
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Steady-Luc™ Firefly HTS assay kit
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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Reagents for Fluor-de-Lys®-Green HDAC fluorometric activity assay kit
Supplier: ENZO LIFE SCIENCES
Cellular assay, Fluor-de-Lys®-green substrate, in 50 mM solution in DMSO
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Apoptosis inducer set
Supplier: ENZO LIFE SCIENCES
The set contains five reagents that induce apoptosis through different mechanisms.
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Blue IHC chromogen (AP), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
Blue IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.
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Myeloperoxidase fluorometric detection kit
Supplier: ENZO LIFE SCIENCES
The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.
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Mitochondria/cytosol fractionation kit
Supplier: ENZO LIFE SCIENCES
For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.
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ATP-Glo™ Bioluminometric cell viability assay
Supplier: Biotium
ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.
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ER-ID™ red assay kit (GFP-CERTIFIED®)
Supplier: ENZO LIFE SCIENCES
ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.
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Steady-Luc™ Firefly HTS assay kit (lyophilised)
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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Solutions colorantes, Ghost Dye™
Supplier: Tonbo Biosciences
Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.
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Apoptosis and necrosis quantitation kits
Supplier: Biotium
Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.
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Catalase fluorometric detection kit
Supplier: ENZO LIFE SCIENCES
The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.
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Blue IHC chromogen (HRP), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
Blue IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.
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Yellow IHC chromogen (HRP), HighDef®
Supplier: ENZO LIFE SCIENCES
Yellow IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.
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Red IHC chromogen (AP), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
Red IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.
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CV-Cathepsin L detection kit
Supplier: ENZO LIFE SCIENCES
Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy.
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ROS-ID™ NO Detection kit
Supplier: ENZO LIFE SCIENCES
The kit is designed to directly monitor real time Nitric Oxide production in live cells by fluorescence microscopy using Nitric oxide Detection Reagent (red fluorescent) as the major component.
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p70 S6K activity kit
Supplier: ENZO LIFE SCIENCES
The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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S-Glutathionylated Protein Detection Kit
Supplier: Cayman Chemical
A convenient method for the direct visualisation of S-glutathionylated proteins in whole (permeabilised) cells.
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qScript® ultra flex kits
Supplier: Quantabio
Faster, longer, tougher next gen cDNA synthesis with flexible priming.
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Black IHC chromogen (HRP), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.
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CV-Caspase 3 & 7 Detection kit
Supplier: ENZO LIFE SCIENCES
Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.
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Apoptosis inducer set
Supplier: G-Biosciences
Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.
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ROS Detection Cell-Based Assay Kit (DHE)
Supplier: Cayman Chemical
Measure superoxide and hydrogen peroxide levels in living cells.
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CV-Cathepsin K detection kit
Supplier: ENZO LIFE SCIENCES
Based on the dye cresyl violet and the cathepsin K LeuArg peptide recognition sequence, this kit allows the detection of cathepsin K activity in living cells by fluorescence microscopy.
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ALDetect™ Lipid Peroxidation Assay kit
Supplier: ENZO LIFE SCIENCES
The assay is designed to measure either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid). It is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole with MDA and 4-hydroxyalkenals to yield a stable chromophore with maximal absorbance at 586 nm.
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LEADING LIGHT® Wnt cell line assay reagents
Supplier: ENZO LIFE SCIENCES
For use with the LEADING LIGHT® Wnt Reporter Cell Line.
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Green long-term cell tracer, CYTO-ID®
Supplier: ENZO LIFE SCIENCES
Live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.
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Supplier: Integra
Supplier: EPPENDORF
Supplier: Integra
Supplier: OZ BIOSCIENCES
Supplier: VWR Collection
Supplier: Corning
Supplier: SenseAnywhere
Supplier: NANOENTEK
Supplier: VWR Collection
Supplier: Integra
Supplier: Testo
Supplier: Sartorius
Supplier: SenseAnywhere
Supplier: Quantabio