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Protein Extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Tryptic Digest of Bovine Serum Albumin

Supplier: BRUKER DALTRONIK

Tryptic digest of bovine serum albumin, for 5×250 spots

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Protein concentration kit, OrgoSol-PROTEIN-Concentrate™

Protein concentration kit, OrgoSol-PROTEIN-Concentrate™

Supplier: G-Biosciences

The OrgoSol-PROTEIN-Concentration™ Kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains the biological activity of most proteins.

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Protein precipitation and concentration kit, UPPA-PROTEIN-Concentrate™

Protein precipitation and concentration kit, UPPA-PROTEIN-Concentrate™

Supplier: G-Biosciences

UPPA PROTEIN-Concentrate™ Kit uses a proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentrate dilute protein samples as low as 1 ng/ml. Concentration is not affected by the presence of common laboratory agents, including detergents and chaotropes. After precipitation, the sample is washed to remove salts and other interfering agents; complete recovery of sample is produced.

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Carrier proteins, Imject™ Maleimide-Activated Blue Carrier™

Supplier: Thermo Fisher Scientific

Imject™ Maleimide-Activated Blue Carrier™ Protein enables conjugation of a sulfhydryl-containing hapten to elicit an immune response and antibody production against the hapten. Blue Carrier™ Protein is a haemocyanin from the Concholepas concholepas mollusc with immunogenic properties similar to keyhole limpet haemocyanin. Blue Carrier™ Protein has a large mass (~8 × 106 Da) and has been successfully used in polyclonal and monoclonal antibody production for
synthetic haptens and peptides.

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Cell surface protein isolation and labelling kit, HOOK™

Cell surface protein isolation and labelling kit, HOOK™

Supplier: G-Biosciences

The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.

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Protein concentration kit, Column-PROTEIN-Concentrate™

Protein concentration kit, Column-PROTEIN-Concentrate™

Supplier: G-Biosciences

The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.

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Sample Grinding Kit

Sample Grinding Kit

Supplier: Cytiva

Sample grinding kit is ready to use for disrupting small tissue and cell samples for protein extraction.

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Washing solution, Protein-OUT™

Washing solution, Protein-OUT™

Supplier: G-Biosciences

Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

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QuEChERS extraction salts, Q-sep®

QuEChERS extraction salts, Q-sep®

Supplier: Restek

QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.

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Uniform latex particles for immobilisation of biomolecules, PL-Latex

Supplier: VARIAN

Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.

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TamiSolve® NxG-PS Solvents for Peptide Synthesis

Supplier: CEM

Modern peptide synthesis agents can dramatically reduce waste generation, but challenges remain regarding the health and environmental impacts of commonly used solvents.

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