You searched for: Protein Extraction

Supplier: Thermo Fisher Scientific

The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.

Supplier: Thermo Fisher Scientific

Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.

Supplier: G-Biosciences

InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.

Supplier: Thermo Fisher Scientific

The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.

Supplier: Thermo Fisher Scientific

Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.

Supplier: Thermo Fisher Scientific

Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.

Supplier: Thermo Fisher Scientific

Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.

Supplier: G-Biosciences

The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.

Supplier: Thermo Fisher Scientific

The In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.

Supplier: Thermo Fisher Scientific

The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.

Supplier: Thermo Fisher Scientific

Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.

Supplier: Thermo Fisher Scientific

Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.

Supplier: Thermo Fisher Scientific

The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.

Supplier: Thermo Fisher Scientific

The Pierce™ In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

Supplier: G-Biosciences

Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

Supplier: Thermo Fisher Scientific

Pierce™ High pH Reversed-Phase Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.

Supplier: MP Biomedicals

Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.

Supplier: Thermo Fisher Scientific

Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

Supplier: Thermo Fisher Scientific

TMT10plex™ Isobaric Mass Tag Labelling Kit and Reagent Sets enable multiplex relative quantitation by mass spectrometry (MS).

Supplier: Thermo Fisher Scientific

The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.

Supplier: Thermo Fisher Scientific

The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

Supplier: Thermo Fisher Scientific

Pierce™ Albumin Depletion Kit improves serum component analysis by rapidly removing abundant albumin protein from serum samples. The albumin depletion resin provided in the kit is a high capacity, immobilised Cibacron Blue dye agarose resin, which binds a variety of species-specific albumins.

Supplier: Thermo Fisher Scientific

The Mitochondria isolation Kit for cultured cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.

Supplier: Thermo Fisher Scientific

This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

Supplier: G-Biosciences

A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Supplier: Thermo Fisher Scientific

SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.

Supplier: Thermo Fisher Scientific

Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.

Supplier: Thermo Fisher Scientific

The glycoprotein isolation kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) or wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples - those containing alpha-lined mannose or terminal glucose residues (in case of ConA) and N-Acetyl glucosamine (GlcNAc) groups or sialic acid (in case of WGA). This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Supplier: G-Biosciences

A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.

Supplier: G-Biosciences

Fraction-FOCUS™ utilises proven technology to fractionate and concentrate all proteomes into multiple fractions; simplifying 2D maps and enhancing detection of low abundant proteins. Fraction-FOCUS™ is fully compatible with all downstream protein identification techniques.