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Protein Extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Bacterial Protein Extraction Kits, B-PER™

Bacterial Protein Extraction Kits, B-PER™

Supplier: Thermo Fisher Scientific

B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

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Complete bacterial protein extraction reagent, B-PER™

Complete bacterial protein extraction reagent, B-PER™

Supplier: Thermo Fisher Scientific

B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

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Total protein extraction kit, TPE™

Total protein extraction kit, TPE™

Supplier: G-Biosciences

Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.

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Membrane protein extraction kit, Pierce™ Mem-PER™ Plus

Membrane protein extraction kit, Pierce™ Mem-PER™ Plus

Supplier: Thermo Fisher Scientific

The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilisation and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.

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FOCUS™ Yeast proteome kits

FOCUS™ Yeast proteome kits

Supplier: G-Biosciences

Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

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Proteome kits, mammalian proteome, FOCUS™

Proteome kits, mammalian proteome, FOCUS™

Supplier: G-Biosciences

Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.

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Proteome kits, FOCUS™ Insect Proteome

Proteome kits, FOCUS™ Insect Proteome

Supplier: G-Biosciences

Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.

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Proteome kits, FOCUS™ Bacterial Proteome

Proteome kits, FOCUS™ Bacterial Proteome

Supplier: G-Biosciences

Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from E.coli, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

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Detergent removal system, DetergentOUT™ GBS10

Detergent removal system, DetergentOUT™ GBS10

Supplier: G-Biosciences

Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

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Protein fractionation kit, FOCUS™ soluble and insoluble

Protein fractionation kit, FOCUS™ soluble and insoluble

Supplier: G-Biosciences

FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.

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Proteome kits, FOCUS™ Plant Proteome

Proteome kits, FOCUS™ Plant Proteome

Supplier: G-Biosciences

Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.

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InGel™ Blue for protein digestion

InGel™ Blue for protein digestion

Supplier: G-Biosciences

InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

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Protein fractionation kit, nuclear and cytoplasmic extraction kit

Protein fractionation kit, nuclear and cytoplasmic extraction kit

Supplier: G-Biosciences

The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.

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Isobaric mass tagging kits and reagent sets, TMT™

Isobaric mass tagging kits and reagent sets, TMT™

Supplier: Thermo Fisher Scientific

Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.

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EpiXtract® total histone extraction kit

EpiXtract® total histone extraction kit

Supplier: ENZO LIFE SCIENCES

Rapid, reliable method to isolate histone proteins in mammalian cells and tissues for histone modification applications.

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Protein fractionation kit, FOCUS™ Glycoprotein

Protein fractionation kit, FOCUS™ Glycoprotein

Supplier: G-Biosciences

FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.

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Detergent removal system, DetergentOUT™ Tween®

Supplier: G-Biosciences

DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

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Carrier proteins

Carrier proteins

Supplier: G-Biosciences

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

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Protein fractionation kit, FOCUS™ Membrane Proteins

Protein fractionation kit, FOCUS™ Membrane Proteins

Supplier: G-Biosciences

FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.

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SilverOUT™, G-Biosciences

SilverOUT™, G-Biosciences

Supplier: G-Biosciences

SilverOUT removes interfering silver ions from gel pieces.

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Biotin resin

Biotin resin

Supplier: G-Biosciences

Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.

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Immobilised Ficin

Immobilised Ficin

Supplier: G-Biosciences

Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.

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Antigen retrieval reagent, IHC

Supplier: ENZO LIFE SCIENCES

Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

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Optimizer blueBALLS™

Optimizer blueBALLS™

Supplier: G-Biosciences

The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.

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Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Supplier: G-Biosciences

Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG1 Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

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EpiXtract® total histone extraction kit (high throughput)

EpiXtract® total histone extraction kit (high throughput)

Supplier: ENZO LIFE SCIENCES

Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.

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FOCUS™ SubCell, protein fractionation kit

FOCUS™ SubCell, protein fractionation kit

Supplier: G-Biosciences

FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.

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EpiXtract® Nuclear protein isolation kit II (nucleic acid-free)

EpiXtract® Nuclear protein isolation kit II (nucleic acid-free)

Supplier: ENZO LIFE SCIENCES

Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.

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Ingel™ for protein digestion

Ingel™ for protein digestion

Supplier: G-Biosciences

InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

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Protein fractionation kit, FOCUS™ signal proteins

Protein fractionation kit, FOCUS™ signal proteins

Supplier: G-Biosciences

Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.

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