Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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RoboSep™ Buffer 2

Supplier: Stemcell Technologies

RoboSep™ buffer 2 is recommended for select EasySep™ cell separation protocols.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Ambion

Running Buffer, TAE buffer solution 25X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: MO BIO

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: Roth Carl

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Molekula

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, PBS solution 25X concentrate (phosphate buffered saline)

Supplier: TREVIGEN

Washing buffer, PBS buffer solution 25X concentrate (phosphate buffered saline)

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Ion pair reagents for analytical HPLC, PIC®

Supplier: WATERS

Tetrabutylammonium hydrogen sulfate, low UV reagent

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Dulbecco's Phosphate Buffered Saline (D-PBS), 10X Concentrate without Ca++ and Mg++

Supplier: Stemcell Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: Ambion

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Molekula

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Dulbecco's Phosphate Buffered Saline (D-PBS) without Ca++ and Mg++

Supplier: Stemcell Technologies

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Ammonium Chloride Solution

Supplier: Stemcell Technologies

Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.

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Buffer, TE solution (TRIS-EDTA buffer) pH 7,5

Supplier: Molekula

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 7,5

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Intracellular Permeabilisation Buffer (10X)

Supplier: Stemcell Technologies

Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.

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ALDEFLUOR™ Assay Buffer

Supplier: Stemcell Technologies

Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.

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Fixation Buffer

Supplier: Stemcell Technologies

Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.