Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
Genomic DNA Isolation Kit, Abcam
Supplier: Abcam
Abcam's Genomic DNA Isolation Kit provides a simple and convenient procedure for rapid isolation of genomic DNA from mammalian cells and tissue samples with high yield and purity.
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Mag-Bind® Universal Pathogen Core Kit
Supplier: OMEGA BIO-TEK
Isolate host genomic, bacterial, fungal spore, and viral DNA and viral RNA from tissue, urine, serum, and fecal samples using magnetic beads.
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Gel extraction kits, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
Gel purification of DNA is a common technique used for the isolation of specific DNA fragments. However, most methods either fail to completely remove agarose (which can lead to problems in downstream manipulations), shear the DNA, or result in very low yields.
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Montage® Gel Extraction Kit
Supplier: Merck
The device utilises gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.
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Chemicon® BacterialXpress™ Nucleic Acid Extraction Kit
Supplier: Merck
BacterialXpress™ Nucleic acid extraction kit is intended for the purification of bacterial DNA from plasma, serum, cerebral spinal fluid, amniotic fluid, tissue, bone marrow, and cell culture.
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E.Z.N.A.® Endo-Free plasmid kits
Supplier: OMEGA BIO-TEK
Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.
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Forensic DNA kit, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Forensic DNA Kit is designed to provide a rapid and easy method for the isolation of genomic DNA from forensic samples such as dry blood, buccal swabs, and semen samples for consistent PCR and Southern analysis. This kit can also be used for the preparation of genomic DNA from mouse tail snips, whole blood, buffy coat, serum, and plasma.
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Bacterial RNA kit, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Bacterial RNA kit is designed for isolation of high-quality total RNA from variety of bacterial strains. Up to 1×10⁹ log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridisation techniques.
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Axygen® AxyPrep® Mag DyeClean kit
Supplier: Corning
Removing excess dye terminator is an essential step prior to Sanger sequencing. Carryover of the excess dye into the sequencing reactions may result in dye blobs resulting in inaccurate results. The AxyPrep™ Mag DyeClean kit utilises a paramagnetic bead-based purification system for Sanger sequencing reaction dye terminator clean-up. The protocol is simple and comprises binding, washing and elution steps which can be performed directly in the thermal cycling plate. This kit requires no centrifugation or filtration steps.
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Mag-Bind® Endo-free Plasmid Midi Kit
Supplier: OMEGA BIO-TEK
Purify endotoxin free (<0,1 EU/µg) plasmid DNA using magnetic beads from up to 50 ml culture volume.
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E.Z.N.A.® SP plant DNA kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® SP plant DNA kit is specially designed for the rapid and reliable isolation of high quality total cellular DNA from a variety of plant species. Up to 100 mg of fresh samples or 30 mg of dry samples can be processed in less than 40 minutes. Purified DNA is suitable for most downstream applications including PCR, restriction digestions and hybridisation techniques.
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Mag-Bind® Total RNA 96 kit
Supplier: OMEGA BIO-TEK
Isolate total cellular RNA and viral RNA from a wide variety of cells and tissues.
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E.Z.N.A.® HP Plant & Fungal DNA Kit
Supplier: OMEGA BIO-TEK
Isolate DNA from plant or fungal sample with organic solvents using spin columns.
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Bisulfite-Seq High Sensitivity Kit (For Illumina®), Abcam
Supplier: Abcam
ab185907 is designed to carry out bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina® platform-based bisulfite sequencing, all in one kit.
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Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA, Abcam
Supplier: Abcam
Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133) enables the user to enrich methylated DNA by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP).
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AdEasy virus purification kit
Supplier: AGILENT
The AdEasy Virus Purification Kit allows the purification and concentration of Adenovirus (from Ad5 strains) with Sartobind® syringe filters containing an ion exchange membrane adsorber that selectively binds adenoviral particles. Once bound, viral particles can be further purified by washing away nonspecifically-bound proteins before elution. Concentrated and purified viral particles can be obtained in 2 to 3 hours, in contrast to traditional CsCl gradient centrifugation which typically takes 12 to 48 hours.
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E.Z.N.A.® blood RNA kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.
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Plasmid isolation, single-strand phage DNA, M-13 isolation kits, E.Z.N.A.® and E-Z 96®
Supplier: OMEGA BIO-TEK
E.Z.N.A.® M13 DNA kits are designed to purify up to 10 μg of single-stranded DNA from up to 3 ml of phage supernatant. Yields of single-stranded DNA obtained using E.Z.N.A.® M13 DNA kits are around 3 to 10 μg and reproducible when the isolations are performed from the same culture. Kits are also available in E-Z 96® DNA plate format.
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Mag-Bind® cfDNA kit
Supplier: OMEGA BIO-TEK
The Mag-Bind® cfDNA Kit is designed for rapid and reliable isolation of circulating DNA from 500 to 4000 µl plasma or serum samples. The Mag-Bind® cfDNA Kit can be processed manually or with automated platforms. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.
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PCR clean-up kit, Axygen® AxyPrep™ Mag
Supplier: Corning
The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.
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Bacterial DNA kit, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
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Universal pathogen DNA kit, Mag-Bind®
Supplier: OMEGA BIO-TEK
The Mag-Bind® Universal Pathogen DNA 96 Kit allows rapid and reliable isolation of high-quality pathogen and host genomic DNA from tissue samples. Up to a 30 mg tissue sample is recommended and can be processed in less than 60 minutes. The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments.
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Mag-Bind® Endo-free Plasmid Mini Kit
Supplier: OMEGA BIO-TEK
The Mag-Bind® Endo-free Plasmid Mini Kit is designed to deliver high-quality, plasmid DNA with endotoxin levels <0,1 EU/μg for use in eukaryotic transfections and other sensitive in vitro applications.
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RNA isolation, total RNA kit, E-Z 96®
Supplier: OMEGA BIO-TEK
The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.
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Mag-Bind® Fit24™ cfDNA Kit
Supplier: OMEGA BIO-TEK
Pre-scripted solution for the purification of cfDNA from up to 4 ml plasma samples.
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E.Z.N.A.® Plasmid DNA Mini Kit I, (Q-spin, capless)
Supplier: OMEGA BIO-TEK
Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.
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Mag PCR normalizer kit, Axygen® AxyPrep
Supplier: Corning
The AxyPrep™ Mag PCR Normalizer Kit utilises a paramagnetic bead-based purification system for PCR clean up.
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DNA Isolation Kit - Urine, Abcam
Supplier: Abcam
The DNA Isolation Kit-Urine (ab156899) is a complete set of essential components which enables the experimenter to efficiently isolate DNA from urine.
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UDI adapters (1-96), sparQ
Supplier: Quantabio
sparQ UDI Adapters (1-96) are unique dual-indexed adapters designed to be used on illumina sequencing platforms. It is compatible with both DNA and RNA libraries. The use of UDI’s minimises index hopping events while also enabling greater multiplexing on flow cells.
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PCR Clean-up Kit, Abcam
Supplier: Abcam
The PCR Clean-up Kit (ab156894) is a complete set of essential components which enables the experimenter to efficiently clean-up PCR products from various sources including conventional or real time thermal cycling PCR and isothermal gene amplification (PCR).