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You searched for: qPCR/RT-qPCR Enzymes and Kits

qScript™ One-Step qRT-PCR kits

qScript™ One-Step qRT-PCR kits

Supplier: Quantabio

Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ Taq DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified Taq DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.

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Verso™ qRT-PCR Kits

Verso™ qRT-PCR Kits

Supplier: Thermo Fisher Scientific

The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

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RT-PCR kits, Verso™

RT-PCR kits, Verso™

Supplier: Thermo Fisher Scientific

The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.

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Probe one-step RT-qPCR kit (No ROX), Luna®

Probe one-step RT-qPCR kit (No ROX), Luna®

Supplier: NEW ENGLAND BIOLABS

In the Luna Probe One-Step RT-qPCR Kit (No ROX), Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition.

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VWR® One-Step RT-qPCR Kit

VWR® One-Step RT-qPCR Kit

Supplier: VWR Chemicals

VWR® One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via dye-based qPCR. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

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VWR® Probe One-Step RT-qPCR Kit

VWR® Probe One-Step RT-qPCR Kit

Supplier: VWR Chemicals

VWR® Probe One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via hydrolysis probes. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

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PerfeCTa® qPCR FastMix® II

Supplier: Quantabio

PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

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VWR®, Real-Time PCR kit for Salmonella spp. in food samples

VWR®, Real-Time PCR kit for Salmonella spp. in food samples

Supplier: VWR Chemicals

Real-Time PCR kit for detection and quantification of Salmonella spp. in food samples. Kit that contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Salmonella spp.

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VWR®, Real-time PCR kit for Legionella spp. (ISO/TS 12869)

VWR®, Real-time PCR kit for Legionella spp. (ISO/TS 12869)

Supplier: VWR Chemicals

Real-Time PCR kit for detection and quantification of Legionella spp. in environmental samples (ISO/TS 12869). Kit contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Legionella spp.

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VWR® Probe One-Step RT-qPCR Master Mix with UDG

VWR® Probe One-Step RT-qPCR Master Mix with UDG

Supplier: VWR Chemicals

VWR® Probe One-Step RT-qPCR Master Mix with UDG is designed for the detection and quantitation of RNA targets and is an ideal choice for multiplex applications. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

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VWR® qPCR Master Mix

VWR® qPCR Master Mix

Supplier: VWR Chemicals

VWR® qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

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VWR®, Real-time PCR kit for Listeria Monocytogenes

VWR®, Real-time PCR kit for Listeria Monocytogenes

Supplier: VWR Chemicals

This kit is designed to detect the pathogen microorganism Listeria monocytogenes in food samples, after enrichment steps, according to standard microbiology procedures. It can also be used to confirm suspect colonies isolated in selective culture media.

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VWR® Probe qPCR Master Mix

VWR® Probe qPCR Master Mix

Supplier: VWR Chemicals

VWR® Probe qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

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qPCR SuperMixes, PerfeCTa®

qPCR SuperMixes, PerfeCTa®

Supplier: Quantabio

PerfeCTa® qPCR SuperMixes are 2X concentrated, ready to use reaction cocktails containing all components except primers, probe and template for qPCR.

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MVP human total RNA

Supplier: AGILENT

MVP RNA is application-ready RNA available in small economical pack sizes.

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Fast probe master mixes

Fast probe master mixes

Supplier: Biotium

A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

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AMPIGENE® qPCR 1-Step Green Kit Hi-ROX

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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Master mixes, brilliant HRM ultra-fast loci

Supplier: AGILENT

The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.

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miRNA QRT-PCR detection reagent

Supplier: AGILENT

Polyadenylation of miRNA from total RNA and synthesis of first-strand cDNA from tailed miRNAs.

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Brilliant probe multiplex MM

Supplier: AGILENT

The brilliant multiplex QPCR master mix allows you to amplify up to four targets in a single real-time PCR Reaction.

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AMPIGENE® qPCR 1-Step Green Kit Lo-ROX

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and dye-based qPCR in a single tube.

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Virus detection kit, qScript™ 1-Step Virus ToughMix®

Virus detection kit, qScript™ 1-Step Virus ToughMix®

Supplier: Quantabio

Superior sensitivity for viral RNA detection.

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PerfeCTa® MultiPlex qPCR SuperMix, low ROX

Supplier: Quantabio

PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.

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Qscript lyo 1-step

Qscript lyo 1-step

Supplier: Quantabio

Qscript lyo 1-step is a lyophilised single-reaction reagent optimised for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes.

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Brilliant II probe MM with ROX

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed to maintain the high quality of original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

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Perfect Match PCR enhancers

Supplier: AGILENT

Increases specificity and yield of primary PCR amplification products.

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PerfeCTa® MultiPlex qPCR ToughMix®

PerfeCTa® MultiPlex qPCR ToughMix®

Supplier: Quantabio

PerfeCTa® MultiPlex qPCR ToughMix® is a 5X concentrated, ready to use reaction cocktail for Real-Time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix® allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta® MultiPlex qPCR ToughMix® has been optimised to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.

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Ultra-Plex™ 1-Step ToughMix® (4X)

Ultra-Plex™ 1-Step ToughMix® (4X)

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

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One-Step SYBR® Green qRT-PCR, qScript™

Supplier: Quantabio

qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

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Brilliant III Ultra-Fast SYBR® green ROX QPCR master mix

Supplier: AGILENT

High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.

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