You searched for: Enzymes

Supplier: Abnova

Synthetic Yeast Inorganic Pyrophosphatase (from E. coli)

Supplier: Abnova

Recombinant mRNA Cap 2'-O-methyltransferase (from E. coli)

Supplier: MP Biomedicals

Aspergillus niger Type X glucose oxidase

Supplier: MP Biomedicals

Soluble in distilled water or dilute buffer; dissolves readily at 5 mg/ml in 0,1 M potassium phosphate pH 7,4.

Supplier: MP Biomedicals

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin.

Supplier: MP Biomedicals

Preparation Method
Produced from 3× crystallised chymotrypsinogen

α-Chymotrypsin is used for treating pancreatic insufficiency and in traumatology.

Chymotrypsin preferentially catalyses the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine, and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. In addition to bonds involving aromatic amino acids, chymotrypsin catalyses at a high rate the hydrolysis of bonds of leucyl, methionyl, asparaginyl, and glutamyl residues. a-Chymotrypsin is a protein consisting of 241 amino acid residues. The molecule has three peptide chains: an A chain of 13 residues, a B chain of 131 residues, and a C chain of 97 residues.

Supplier: VIAGEN BIOTECH

Proteinase K from the fungus engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active.

Supplier: Merck

ADH (alcohol dehydrogenase) is a enzymes for biofuel cell research.

Supplier: ANKOM TECHNOLOGY

This enzyme formulation was developed specifically for use in the ANKOMTDF dietary fiber analyser. With all of the necessary ingredients in this concentrated formula, just add the correct amount of water and the enzyme solution is ready for use in the instrument. This product has enough enzyme for approximately 500 ANKOMTDF dietary fiber analyser assays.

Supplier: Merck

SOLu-Trypsin is an advanced proteomics grade enzyme that is solution stable for mass spectrometry. Recombinant, expressed in Pichia pastoris.

Supplier: ANKOM TECHNOLOGY

This enzyme formulation was developed specifically for use in the ANKOMTDF dietary fiber analyser. With all of the necessary ingredients in this concentrated formula, just add the correct amount of water and the enzyme solution is ready for use in the instrument. This product has enough enzyme for approximately 500 ANKOMTDF dietary fiber analyser assays.

Supplier: Merck

Ascorbate Oxidase is an enzyme belonging to the family of oxidoreductases that catalyses the reaction: Ascorbic acid + ½ O₂ ascorbate oxidase → Dehydroascorbic acid + H₂O.

Supplier: Merck

Cellulase, enzyme blend contains cellulases, ß-glucosidases, and hemicellulase, for the application of degrading cellulose to fermentable sugars.

Supplier: MEGAZYME

This purified fructanase mixture is used for the determination of fructn (liquid), in research, biochemical enzyme assays and in vitro diagnostic analysis.

Supplier: Merck

Butyrylcholinesterase from equine serum has been used in a microcalorimetric study of the inhibition of butyrylcholinesterase by paraoxon. This enzyme has also been used in a study to investigate the synthesis and inhibition of cholinergic enzymes.

Supplier: Merck

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7,4, 150 mM NaCl at 10 mg/ml.

Supplier: Merck

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Supplier: MEGAZYME

High purity exo-1,3-β-D-Glucanase (Trichoderma sp.) + β-Glucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Supplier: Merck

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Supplier: Merck

Aspergillus oryzae α-amylase enzyme catalyzes the hydrolysis of the α-1,4 glycosidic bonds in soluble starches and related subsrates. These substrates are broken down to release short oligosaccharides and α-limit dextrins. Also act as control enzyme in agar plate-based and carboxymethylcellulose-based clearing assays to screen cellulase activity.

Supplier: Merck

Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm. It catalyses the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway. Also it is a key regulatory enzyme in the first step of the pentose phosphate pathway.

Supplier: Merck

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8,75. It selectively hydrolyses peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, α2-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Supplier: C LECTA

DENARASE High Salt is an engineered version of the wild-type Serratia marcescens endonuclease, which is commonly used for DNA removal applications in bioprocessing. Compared to the wild-type DENARASE, a few amino acids were substituted to provide the enzyme with an improved salt tolerance without losing its specificity for nucleic acids.

Supplier: Merck

Aldehyde dehydrogenase from baker′s yeast catalyses the reduction of pyridine nucleotides by several aldehydes. It catalyses the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. The enzyme has been used to study the production of ethanol and isobutanol.

Supplier: Merck

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.The enzyme is active over a broad pH range (6,0 to 9,0). At pH 6,2, maximal activity is observed over a wider range of ionic strengths (0,02 to 0,100 M) than at pH 9,2 (0,01 to 0,06 M).

Supplier: Merck

Hexokinase

Supplier: Merck

Recombinant desoxyribonuclease I

Supplier: Merck

L-Lactate dehydrogenase

Supplier: Merck

Horseradish peroxidase, Typ X

Supplier: Merck

Linamarase (beta-D-Glucosidase)