Enzymes

Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.

Pig Pepsin (from Stomach)

Supplier: SIGMA ALDRICH MICROSCOPY

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.

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Histo/Zyme

Supplier: SIGMA ALDRICH MICROSCOPY

Histo/Zyme is a stabilized proteolytic enzyme in buffered solution, used to unmask immunoreactive sites altered by fixation and/or the embedding process. Treatment with a proteolytic enzyme in immunohistochemistry (IHC) is required to digest excess linkages and expose the antigen. The use of Histo/Zyme results in superior staining over traditionally used enzyme pretreatments, such as pepsin and trypsin. Moreover, Histo/Zyme requires only a brief incubation at room temperature. Performing antigen recovery with Histo/Zyme will allow you to dilute the primary antibody 5 to 10 fold further and achieve optimal staining.

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VWR®, Aspergillus Glucose oxidase

Supplier: VWR Chemicals

Beta-D-glucose oxidase with high purity grade and storage in frozen condition.

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VWR®, Ficin

Supplier: VWR Chemicals

Source is Fig Latex. Fine, light beige powder

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VWR®, Pepsin 1:10000

Supplier: VWR Chemicals

Pepsin is a proteolytic enzyme product derived from porcine stomach linings.

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Bovine Thrombin (from Plasma)

Supplier: SIGMA ALDRICH MICROSCOPY

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

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J.T.Baker® Endonuclease

Supplier: Avantor

J.T.Baker® Endonuclease Biotech Reagent meets the strictest cGMP standards and is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Biotech Reagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease Biotech Reagent is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. Absence of proteolytic activity. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Biotech Reagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.

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J.T.Baker® Endonuclease

Supplier: Avantor

J.T.Baker® Endonuclease Ultrapure Bioreagent is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Ultrapure Bioreagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Ultrapure Bioreagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.

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VWR® Trichoderma viride Cellulase

Supplier: VWR Chemicals

10,000 units is that quantity of enzyme which will degrade 2 cm² of cellulose filter paper (17 mg) in 1 minute in 0,1 M acetate buffer at pH 4,0.

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VWR®, Pronase E

Supplier: VWR Chemicals

Off-white to light brown powder from Streptomyces griseus.

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Proteinase K solution

Supplier: PanReac AppliChem

Proteinase K is used to destruct proteins in cell lysates (tissue, cultured cells) and to liberate nucleic acids, since it very effectively digests DNases and RNases.