"mRNA"

Supplier: OMEGA BIO-TEK

The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® tissue DNA kit offers a simple, rapid, and cost effective method for the isolation of DNA from a wide variety of sample sources including fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in less than 30 minutes. Up to 30 mg of tissue at a time can be readily processed at a time using the simple E.Z.N.A.® tissue DNA protocol. Single or multiple samples can be simultaneously processed with this spin-column based kit. There is no need for phenol/chloroform extractions, or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.® tissue DNA kit is ready for most downstream applications such as PCR, southern blot and restriction enzyme digestion.

Supplier: MACHEREY-NAGEL

Additional supplies and consumables for DNA and RNA extraction kits.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

Supplier: MP Biomedicals

MPure™ FFPE DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for the extraction and purification of genomic DNA from FFPE (formalin-fixed, paraffin-embedded) tissue samples, providing high quality, high integrity DNA for molecular diagnostics and research use.

Supplier: MP Biomedicals

MPure™ Forensic DNA Extraction Kit is used with the MPure-12™ aNAP system for the extraction and purification of genomic DNA from forensic samples.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Supplier: MP Biomedicals

MPure™ Viral Nucleic Acids Extraction Kit is used with the MPure-12 instrument for extraction of Viral DNA or RNA from human biological specimens such as serum, plasma, and other cell-free fluids.

Supplier: Corning

Axygen power supplies are ideal for most electrophoresis needs.

Supplier: MACHEREY-NAGEL

NucleoMag kits are designed for rapid manual and automated small-scale preparation of viral RNA and DNA and the DNA of microorganisms from various types of clinical samples. NucleoMag® kits allow easy automation on common liquid handling instruments or automated magnetic separators.

Supplier: MP Biomedicals

The GNOME® DNA Isolation Kit is a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types. No organic extractions are required, and up to 100 μg of DNA can be isolated per prep.

Supplier: MP Biomedicals

MPure™ Viral/Pathogen Nucleic Acids Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for purification of viral DNA/RNA and pathogen DNA from swab samples (cell-rich samples).

Supplier: MP Biomedicals

The GeneClean® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels.

Supplier: MACHEREY-NAGEL

Combining selective purification technologies and chemistries, a comprehensive range of specialized DNA isolation kits for a broad range of diversified starting materials. The resulting high quality DNA serves as excellent basis for following demanding downstream applications.

Supplier: Corning

The AxyPrep™ Mag Plasmid purification kit utilises a magnetic bead technology for high-throughput purification of plasmid DNA from E.coli cells.

Supplier: EKSIGENT

The DNA 20 kb plasmid and linear kit is used to do isoform and purity analysis of plasmid and linearised dsDNA.