"derivatisation reagent"
1,1,1,3,3,3-Hexamethyldisilazane : Chlorotrimethylsilane (HMDS + TMCS) (2:1) Silyl-21 silylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
Silyl-21 reagent will derivatise amides and many secondary amines and hindered hydroxyls that would not be completely derivatised by HMDS alone.
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1,1,1,3,3,3-Hexamethyldisilazane : Chlorotrimethylsilane (HMDS + TMCS) (2:1:10) in pyridine Silyl-2110 silylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
Silyl-2110 reagent will derivatise alcohols, bile acids, phenols, most steroids, sterols, and sugars that would not be completely derivatised by HMDS alone.
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N,O-Bis(trimethylsilyl)acetamide silylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
BSA is a strong silylation reagent that forms very stable TMS derivatives with a large variety of compounds, e.g., non-sterically hindered alcohols, carboxylic acids, phenols, enols, steroids, (biogenic) amines and alkaloids.
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Chlorotrimethylsilane silylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
TMCS is often used as a catalyst with other trimethylsilyl reagents. Without additives it can be used for preparing TMS derivatives of organic acids. Together with methanol it can be used for methylation.
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Dimethyldichlorosilane silylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
DMCS is used to form dimethylsilyl (DMS) derivatives. These derivatives are much more susceptible to hydrolysis than TMS derivatives. Therefore strictly anhydrous conditions during reaction are very important.
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Pentafluorobenzoyl chloride acylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
Pentafluorobenzoyl chloride (PFBC) will react with hydroxyls, primary and secondary amines, amides and thiols.
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1-(Trimethylsilyl)imidazole silylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
TSIM is the strongest hydroxyl silylator; reagent of choice for carbohydrates and most steroids (even highly hindered steroids).
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Heptafluorobutyric anhydride (HFAA) acylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
Acylation with HFBA can be used for alcohols, phenols, carboxylic acids, amines, amino acids and steroids forming volatile, stable derivatives suited for GC-FID as well as for GC-ECD detection.
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Trifluoroacetic anhydride acylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
Acylation with fluorinated acid anhydrides can be used for alcohols, phenols, carboxylic acids, amines, amino acids and steroids forming volatile, stable derivatives suited for FID as well as for ECD detection.
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N-Methyl-bis-trifluoroacetamide acylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
This derivitisation reagent is recommended for alcohols, primary and secondary amines as well as for thiols under mild, neutral conditions. MBTFA also forms very volatile derivatives with carbohydrates.
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Trimethylsulphonium hydroxide 0.2 M in methanol alkylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
Methylation with TMSH is recommended for free acids, chlorophenoxycarboxylic acids, their salts and derivatives as well as for phenols and chlorophenols. Lipids or triglycerides can be converted to the corresponding fatty acid methyl esters (FAMEs) by a simple transesterification.
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N-Methyl-N-trimethylsilylheptafluorobutyramide (MSHFBA) silylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
Silylation reagent with recommended applications: carboxylic acids, alcohols, phenols, primary and secondary amines and amino acids.
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N-Methyl-N-(trimethylsilyl)trifluoroacetamide silylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
Silylation in GC stands for replacement of active hydrogen atoms by a trimethylsilyl group (TMS derivative).
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HMDS (1,1,1,3,3,3-hexamethyldisilazane) silylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
HMDS is weak TMS donor. If used as sole reagent, it is slow and not very effective. After addition of catalytic quantities (e.g., 1 %) of TMCS or as a mixture with TMCS it is a fast and quantitative reagent for trimethylsilylation of organic compounds.
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N-Methyl-bis-heptafluorobutyramide (MBHFBA) acylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
Acylation with fluorinated bisacylamides is recommended for alcohols, primary and secondary amines as well as for thiols under mild, neutral conditions.
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N-(tert-Butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) silylation reagent, for GC derivatisation
Supplier: MACHEREY-NAGEL
MBDSTFA is a silylation reagent that donates a tert-butyldimethylsilyl group (TBDMS) for derivatizing active hydrogen atoms in hydroxyl, carboxyl and thiol groups, primary and secondary amines as well as amino acids. Fast reactions (typically 5–20 min) with high yields (> 96 %). By-products are neutral and volatile.