Exonuclease III

Supplier: USB MOLECULAR BIOLOGY REAGENTS

Exonuclease III, the major apurinic/apyramadinic (AP) endonuclease in E. coli, is a 3' to 5' exonuclease digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex. Exonuclease III breaks phosphodiester bonds on the 5' side of AP sites in both dsDNA and ssDNA, removes 3'mterminal groups on dsDNA, increases MutY turnover, and efficiently degrades 3' recessed but not 3' protruding DNA ends (creating 5' overhangs). These properties have been exploited to allow strand specific labelling, unidirectional deletions, and selective strand degradation.

Expand 2 Items

RNase I

Supplier: USB MOLECULAR BIOLOGY REAGENTS

RNase I catalyses the hydrolysis of 3' to 5' phosphodiester bonds of RNA with the formation of oligoribonucleotides terminating in pyrimidine 2' to 3' cyclic phosphate. RNase I is a preparation of 70% RNase A and the remaining 30% is other isozymes of RNase.

Expand 1 Items

Glucose oxidase

Supplier: USB MOLECULAR BIOLOGY REAGENTS

Glucose oxidase

Expand 2 Items

Hexokinase

Supplier: USB MOLECULAR BIOLOGY REAGENTS

Hexokinase

Expand 3 Items

Bovine RNase I 'A' (from Pancreas)

Supplier: USB MOLECULAR BIOLOGY REAGENTS

Source: Bovine pancreas.

Expand 1 Items

ExoSAP-IT® reagent for PCR product clean up

Supplier: USB MOLECULAR BIOLOGY REAGENTS

ExoSAP-IT® reagent is designed for simple, quick PCR clean up for downstream applications, such as DNA sequencing or Single Nucleotide Polymorphism (SNP) analysis. When PCR amplification is complete, any unconsumed dNTPs and primers remaining in the PCR product mixture will interfere with these methods. ExoSAP-IT® removes these contaminants.

Expand 5 Items

Trypsin

Supplier: USB MOLECULAR BIOLOGY REAGENTS

Trypsin

Expand 2 Items

Malate dehydrogenase

Supplier: USB MOLECULAR BIOLOGY REAGENTS

Malate dehydrogenase

Expand 1 Items

T4 endonuclease VII

Supplier: USB MOLECULAR BIOLOGY REAGENTS

T4 Endonuclease VII is a DNA junction specific endonuclease (also referred to as a resolvase or a cleavase). T4 Endonuclease VII is the product of gene 49 of phage T4. This gene product has 157 amino acid residues and a mass of 18 kDa. T4 Endonuclease VII functions in vivo to resolve branched DNA structures in newly synthesised DNA. It does this by cleaving the DNA flanking the branch prior to rearrangement and reannealing of the DNA strands.

Expand 1 Items

Thrombin

Supplier: USB MOLECULAR BIOLOGY REAGENTS

Thrombin

Expand 1 Items

Trypsin

Supplier: USB MOLECULAR BIOLOGY REAGENTS

Trypsin

Expand 1 Items

Proteinase K

Supplier: USB MOLECULAR BIOLOGY REAGENTS

Proteinase K is a highly active and stable endopeptidase used in a wide range of applications purified from the fungus T. album. It cleaves peptide bonds mainly following the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids and is classified as a serine protease. Proteinase K is useful in purifying high molecular weight nucleic acids from cells and tissues and in revealing protein structure and function.

Expand 1 Items

Shrimp recombinant Dnase

Supplier: USB MOLECULAR BIOLOGY REAGENTS

Shrimp DNase is an endonuclease that cleaves phosphodiester linkages in DNA to yield di- and oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. This DNase has a remarkably high specific activity towards double-stranded DNA (dsDNA). The activity towards dsDNA is 5000-fold higher than towards single-stranded DNA, and thus can be used selectively to degrade dsDNA, leaving single-stranded DNA intact. The activity of this enzyme depends on Mg²⁺ concentration and is stimulated by Ca²⁺. However, Ca²⁺ also stimulates the RNase activity of shrimp DNase and should be avoided when RNA integrity is critical.

Expand 2 Items

T4 DNA ligase

Supplier: USB MOLECULAR BIOLOGY REAGENTS

T4 DNA Ligase is an ATP-dependent enzyme that catalyses the formation of a phosphodiester bond between juxtaposed 5' phosphoryl and 3'-hydroxyl termini in duplex DNA or RNA. T4 DNA Ligase repairs single-stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids. T4 DNA ligase will join both blunt-end and cohesive-end fragments of duplex DNA or RNA(1), making it commonly used in cloning of restriction fragments and ligation of synthetic linkers or adapters.

Expand 5 Items

DNase I

Supplier: USB MOLECULAR BIOLOGY REAGENTS

DNase I is an endonuclease that catalyses the degradation of both single and double-stranded DNA to yield di-, tri-, and oligonucleotides with 5'-phosphate and 3'-hydroxyl termini. DNase I is stabilised by the addition of Ca²+ and requires a divalent metal cation for activity. It randomly produces nicks in double-stranded DNA in the presence of Mg²+, but in the presence of Mn²+, both strands of double-stranded DNA are cleaved leaving blunt-end fragments or those with one or two protruding nucleotides.