38 Results for: "Tissue Culture Plastics"

Supplier: MP Biomedicals

PlantCon™ container is a presterilised, disposable, plastic growth containers. It consists of a cover and a bottom, and features a vent for gas exchange, and a drip control lip to recycle condensation.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: VWR Collection

Pipettes made from PS for sterile serological and tissue culture applications.

Supplier: VWR Chemicals

Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.

Supplier: VWR Collection

Adhesive sealing film or foil rolls constructed on plastic cores, for use with high throughput automated microplate sealers.

Supplier: VWR Chemicals

Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)

Supplier: VWR Chemicals

Cell culture buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Ribonuclease A is an endoribonuclease that efficiently hydrolyses RNA from tissue or bacterial cell cultures.

Supplier: VWR Chemicals

Dulbecco's formulation without calcium and magnesium.

Supplier: Corning

Polyester mesh bottoms attached to PS rings or housing. Netwell™ inserts are used as tissue carriers, supports and strainers of small organs, tissue slices or explants at the air-media interface.

Supplier: VWR Chemicals

137 mM sodium chloride, 2,7 mM potassium chloride, 12 mM phosphate buffer

Supplier: VWR Collection

Ideal for cell growth and cell yields.

Supplier: Corning

The bottle is made with one piece seamless construction, has a wide mouth and printed graduations.

Supplier: Corning

PC, square.

Supplier: Corning

Synthemax® II-SC Substrate is an easy to use, self-coating material for creating a unique synthetic surface that mimics the natural cell environment. This surface supports the adhesion and expansion of stem cells in their undifferentiated state and directs differentiation into specialised cell types. In addition, it offers a synthetic, xeno-free alternative to biological coatings and/or feeder cell layers used in hES and iPS cell culture, whenever needed and appropriate.

Supplier: VWR Collection

PS, PC or PETG, with PP screw cap, sterile.

Supplier: VWR Collection

VWR® multiwell cell culture plates are available with a choice of surface treatments.

Supplier: VWR Chemicals

PBS, Ultra Pure Grade is a powdered, magnesium- and calcium-free formulation of PBS suitable for cell culture and for use in flow cytometry applications.

Supplier: KIMBLEKONTES

Manufactured from 51 expansion borosilicate glass, KIMAX®.

Supplier: KIMBLEKONTES

Manufactured from 51 expansion borosilicate glass.

Supplier: KIMBLEKONTES

Borosilicate glass, disposable, sterile. These pipettes are ideal for use in tissue culture work and other procedures performed under a fume hood or in other confined areas.

Supplier: VWR Collection

VWR® cell slides are made from medical grade, high transparency PET, which has high strength and is unbreakable. Only the surface of the cell slide has a hydrophilic treatment so adherent cells grow easily, whilst the surrounding culture dish surface is hydrophobic, making it difficult for adherent cells to grow.

Supplier: Ludwig Schneider GmbH & CO. KG

These thermometers are used for special applications.

Supplier: PAN BIOTECH

Collagen is one of the main structural components of the ECM in connective tissue and internal organs. It is mostly prevalent in skin, tendon, and bones. Type I Collagen is a heterodimer consisting of two α₁(I)-chains and one α₂(I)-chain, that spontaneously form a triple helix at neutral pH and 37 °C.

Supplier: TPP

Designed for gentle removal and harvesting of tissue cultures in all types of flasks, dishes and tubes.

Supplier: Corning

PS, transparent, graduated, sterile.

Supplier: Corning

Corning® PET media bottles are equipped with a screw cap designed to ensure leak-proof performance.

Supplier: HEATHROW

Pipette rack made of blue ABS plastic.

Supplier: DRUMMOND

The Pipet-Aid® was designed to transfer liquids with the use of serological, volumetric and/or blow-out pipettes in either glass, plastic or something similar. It accepts 1 to 100 ml pipettes.

Supplier: VWR Collection

Typical applications are clarification, sterile filtration, sample preparation and sterile venting.