"Staining Solutions"

Supplier: EDVOTEK

Genetic testing can be used to identify people with a genetic condition which caused them to have an elevated level of cholesterol and which can be fatal. Students can see how genetic testing is carried out and learn about DNA electrophoresis.

Supplier: MP Biomedicals

2,3,5-Triphenyltetrazolium Chloride is a redox indicator. In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains as white TTC in areas of necrosis since these enzymes have been either denatured or degraded.
2,3,5-Triphenyltetrazolium Chloride is commonly used in biochemical experiments especially to indicate cellular respiration. TTC has been employed in autopsy pathology to assist identification of post-mortem myocardial infarctions. Healthy viable heart muscle will stain deep red from the cardiac lactate dehydrogenase; while areas of potential infarctions will be more pale. Useful indicator for reducing substances

Supplier: Thermo Fisher Scientific

External-controlled hotplates with porcelain-coated stainless-steel top.

Supplier: MP Biomedicals

Storage: +4°C, protect from light
3- (4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Supplier: MP Biomedicals

Coomassie® Brilliant Blue R-250 is a fast acting, sensitive dye, which can be used on SDS gels, IEF gels, and standard gels.

Supplier: MP Biomedicals

Agarose is a natural polysaccharide isolated from agar or agar-bearing marine algae. Its physical and chemical properties are particularly suited for use as a gel medium for diffusion and electrokinetic movement of biomolecules. Agarose is essentially biologically inert and forms relatively clear, transparent gels. Agarose based gels offer several advantages: High gel strength permits use of low concentrations. The macroporous nature of the gels allows rapid diffusion of high molecular weight molecules. Agarose is Non-toxic. Agarose gels are thermally reversible facilitating sample recovery. Staining and destaining may be done rapidly with minimal background. Because agarose forms macroporous gels that are electrically nonionic, it is an excellent medium for Isoelectric Focusing (IEF). A selection of different formulated agaroses with specific qualities for unique applications are available from MP.
Multipurpose Agarose is a versatile high gel strength agarose, especially designed for a wide range of molecular biology techniques. It is used for resolution of fragments over a large size range from 200 bp up to 50 kb by conventional electrophoresis. It is suitable for use in pulsed field gel electrophoresis and for blotting assays.
High gel strength agarose, especially designed for a wide range DNA. Versatile – separate fragments from 200 bp up to 50 kb by conventional electrophoresis. Concentrations between 0.4% and 6%. Multipurpose Agarose features the highest gel strength available, allowing 0.4 - 0.5% agarose gels. Significantly decreases electrophoresis times. Improves separation of very large fragments. Dissolves easily in a microwave.

Supplier: RANKEM

FIELD STAIN A SOLUTION 1 * 500 mL

Supplier: RANKEM

HAEMATOXYLIN STAIN SOLUTION 1 * 125 mL

Supplier: RANKEM

GIEMSA STAIN SOLUTION 1 * 500 mL

Supplier: RANKEM

FIELD STAIN B SOLUTION 1 * 500 mL

Supplier: EKSIGENT

The DNA 20 kb plasmid and linear kit is used to do isoform and purity analysis of plasmid and linearised dsDNA.

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.