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"Staining Solutions"

 
 
VWR® Q Path® Isomount 2000, Mounting Media

VWR® Q Path® Isomount 2000, Mounting Media

Supplier: VWR Chemicals

Mounting media based on isopropyl alcohol. Compatible with most solvent replacing agents. Suitable for manual and automatic mounting of slides. PET bottle of 500 ml.

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Automated cell counter, ADAM™ MC2

Automated cell counter, ADAM™ MC2

Supplier: NANOENTEK

ADAM™ MC2 is an automated fluorescence cell counter that performs cell counting and viability measurements at highest accuracy capable of replacing complex flow cytometry workflows. Combined with the disposable micro-fluidic AccuTip, operation is extremely simple and cost-effective. Utilizing the PI (Propidium Iodide) nucleus staining method, most advanced LED optics and CMOS detection technology, ADAM-MC2 is the perfect solution when working with aggregated, irregular shaped or challenging cells like CAR-T cells, CAR-NK cells or adipose-derived stem cells.

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VWR®, OCT Embedding matrix for frozen sections

VWR®, OCT Embedding matrix for frozen sections

Supplier: VWR Chemicals

When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.

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VWR®, Embedding compound, water- soluble

VWR®, Embedding compound, water- soluble

Supplier: VWR Collection

Frozen section compound is a fast-acting, water-soluble embedding compound designed for use in frozen sectioning. The compound bonds and encapsulates tissue specimens to the object holder for cryosectioning. At a working temperature of –20 °C, it provides optimal sectioning consistency with minimal section curling.

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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™

Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™

Supplier: Biotium

EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

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VWR® Q Path® Coverquick, Mounting Agents for Microscopy

VWR® Q Path® Coverquick, Mounting Agents for Microscopy

Supplier: VWR Chemicals

A range of mounting media for manual and automatic mounting of slides. The Q Path® Coverquick range consists of four different mounting media with different viscosities to meet all microscopy needs. The number after the name indicates increasing viscosity: Q Path® Coverquick 1000 has the lower viscosity (around 260 mPaS) and Q Path® Coverquick 4000 has the higher viscosity (around 920 mPaS).

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Bouin liquor

Bouin liquor

Supplier: PanReac AppliChem

Fixative for preserving soft and delicate structures, used as a mordant in various trichrome procedures.

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Immersion oil, BioChemica

Immersion oil, BioChemica

Supplier: PanReac AppliChem

Biochemical grade immersion oil for microscopy.

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Immersion oil

Immersion oil

Supplier: PanReac AppliChem

This fluorescence-free immersion oil for microscopy is highly UV-permeable and free of PCB's.

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SYBR® Green I staining reagent DNA-free

SYBR® Green I staining reagent DNA-free

Supplier: PanReac AppliChem

SYBR Green is an cyanine dye that acts for the detection of double stranded DNA. This ten fold concentrated dye solution is suitable for qPCR when primers such as 16S rDNA or 18S rDNA primers are used.

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FLUO-RAL Kit Fluorescent Staining

Supplier: RAL

Kit Fluo-RAL offers a rapid method for the identification of mycobacteria or Acid Fast Bacilli (AFB). The penetration by the discolouring agent of the highly specific structure of the wall is hardly possible.

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Histofix® – Formalin Preservative (CE-IVD) for Clinical Diagnostics, ITW Reagents

Supplier: PanReac AppliChem

Histofix® is a ready-to-use formaldehyde-based fixative, buffered and stabilized for optimal tissue preservation. Designed for clinical laboratories, it complies with Regulation (EU) 2017/746 on in vitro diagnostic medical devices (IVDR), ensuring safety, consistency, and diagnostic reliability.

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Eosin Y (yellowish) solution 10%

Supplier: MP Biomedicals

Eosin Y disodium salt is used in combination with Azure II to show morphological details of marrow cells; buffered Azure A/Eosin Y method for demostrating cell granules, microorganisms and nuclei; for showing insect musculature; for demonstrating cells infected with mosquito-iridescent virus; use in combination with Alum Hematoxylin/Azure II to show mast cells, early necrobiotic changes in cells and protozoans, rickettsias and bacteria in tissues; to demonstrate volutin granules in any microorganism; Bond's modification of Mann's Eosin-Methyl Blue for Negri bodies in smears; Harris stain for Negri bodies in smears; Mann's method for staining Negri bodies in sections; Gridley's stain for Endamoeba in tissue sections; a modified methenamine-silver nitrate method for the demonstration of Endamoeba in sections. It is used as a counterstain for hematoxylin.

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Cleaning solution, heavy-duty, MegaClean™

Cleaning solution, heavy-duty, MegaClean™

Supplier: MICRONOVA

A non-flammable, heavy-duty cleaner and degreaser that removes grease, adhesives, and photo-resistant stains from all general surfaces. Filtered to 0.1 µm, this cleaner is ideal for semiconductor, electronics, aerospace, and clean-build operations.

   Sustainable Options Available
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Vectashield® mounting medium

Supplier: VECTOR LABORATORIES

Vectashield® Mounting Medium is a unique, stable formula for preserving fluorescence. It is superior to other anti-fading reagents or mounting media for fluorescence microscopy. Some agents retard the rate of fading, but their initial application gives a severe reduction in emission. Others give a good initial reading but bleach upon illumination. Vectashield® Mounting Medium combines both advantages into one product. It provides strong initial fluorescence and retards photobleaching over time. Vectashield® Mounting Medium is supplied in a bottle fitted with a cone-seal cap. A cap fitted with a drop dispenser pipette is also included. The cone-seal cap can be replaced with the drop dispenser cap for routine use and during storage. Mounted slides should be stored at 4 °C. The fluorescence will remain for many weeks.

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Sudan IV, red brown, powder C.I. 26105

Supplier: MP Biomedicals

This product is soluble in chloroform (10 mg/ml), toluene, methanol. Saturated solutions can be prepared in isopropanol. Insoluble in water.

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