FLUO-RAL Kit Fluorescent Staining
Supplier: RAL
Kit Fluo-RAL offers a rapid method for the identification of mycobacteria or Acid Fast Bacilli (AFB). The penetration by the discolouring agent of the highly specific structure of the wall is hardly possible.
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Erythrosin B, red powder C.I. 45430:2
Supplier: MP Biomedicals
Erythrosin B is a red, anionic xanthene dye.
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Cleaning solution, ES 7X™, MP Biomedicals
Supplier: MP Biomedicals
Safe and effective cleaning solution for laboratory instruments and plastic, glassware.
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Trypan blue 0,4% in PBS, dark blue solution
Supplier: MP Biomedicals
Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxicity and proliferation assays.
Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system.
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Pararosaniline hydrochloride ≥88% (dye content), crystalline powder C.I. 42500
Supplier: MP Biomedicals
Basic Fuchsin is a cationic triphenylmethane dye.
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Dishes, aluminium, flat bottom, with cover
Supplier: Thermo Fisher Scientific
Dishes, Aluminium, flat bottom, straight wall, with cover.
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3,3',5,5'-Tetramethylbenzidine (TMB) membrane peroxidase substrate Plus, liquid, Biotechnology Grade 1 component
Supplier: VWR Chemicals
Ready to use sensitive substrate for the detection of horseradish peroxidase (HRP) activity suitable for ELISA and solution assays. Offers faster signal, enhanced sensitivity and the lowest possible background.
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Dishes, aluminium, flat bottom, straight wall
Supplier: Thermo Fisher Scientific
Dishes, Aluminium, flat bottom, straight sides.
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Safranine O, dark green powder C.I. 50240
Supplier: MP Biomedicals
Safranine O is a redox and adsorption indicator.
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bisbenzimide H-33342 trihydrochloride (Hoechst 33342), light yellow powder
Supplier: MP Biomedicals
A membrane permeable, low cytotoxic fluorescent supravitol dye, reversibly binding to DNA, staining the AT-rich regions of the double stranded DNA. It has been shown to displace several known DNA intercalators. Adriamycin interferes with dye uptake.
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3,3',5,5'-Tetramethylbenzidine (TMB), liquid, Biotechnology Grade 1 component
Supplier: VWR Chemicals
Ready-to-use sensitive substrate for the detection of horseradish peroxidase activity
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Coomassie brilliant blue G-250, dark reddish purple powder for electrophoresis C.I. 42655
Supplier: MP Biomedicals
Brilliant blue G is a useful dye for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualised during staining. It has been used in the Bradford dye-binding protein assay.
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Rose Bengal, dark red powder
Supplier: MP Biomedicals
Rose Bengal is a fluorescent probe for determining NAD binding sites of alcohol dehydrogenase.
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Saponin (from plant), white powder, Practical grade
Supplier: MP Biomedicals
Saponin is "built" from quillaic acid, a sugar and possibly other substances. It is a Glycoside widely distributed in plants.
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3,3',5,5'-Tetramethylbenzidine (TMB) ≥99%, powder
Supplier: MP Biomedicals
3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620 to 650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases. It is useful in gel staining procedure for low levels of home-associated peroxidases and for enzyme immunoassay of horseradish peroxidase.
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VWR® Mini Blot, 3-D Shaker
Supplier: VWR Collection
The Mini blot shaker combines the actions of a rocker and a shaker to produce a three-dimensional motion that is gentle enough for fragile gels and blots, yet provides thorough mixing.
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4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride), yellow powder
Supplier: MP Biomedicals
4',6-Diamidino-2-phenylindole Dihydrochloride is a cell permeable fluorescent minor groove-binding probe for DNA.
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Indocyanine green ~90% (dye content)
Supplier: MP Biomedicals
Indocyanine green (ICG) is a cyanine dye used in medical diagnostics.
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Sudan III, redish brown powder C.I. 26100
Supplier: MP Biomedicals
Sudan III is used in Coloring oils, sprit, lacquers etc; also as stain for zoological, pathological and vegetable objects like wax, cutin, resin, contents of lactiferous ducts etc, which are colored red, while cellulose membranes remain uncolored. Approved by FDA for external use only.
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Coomassie® brilliant blue R-250, purple powder C.I. 42660
Supplier: MP Biomedicals
Coomassie Blue R-250 is a triphenylmethane dye used extensively in SDS-PAGE for the analysis of proteins.
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Retriever thermal slide processor
Supplier: ELECTRON MICROSCOPY SCIENCES
Run antigen unmasking in two various buffers at once.
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VWR® Basic, Gel Documentation System
Supplier: VWR Collection
The VWR® Basic gel documentation system offers reproducible, high resolution digital images for gel documentation. This entry level system is easy to use and allows real time images to be captured and viewed directly on a PC or laptop (not supplied). The VWR® Basic system can be illuminated using transillumination from UV, white or blue light. These flexible lighting options make the VWR® Basic suitable for capturing and viewing images from a wide range of samples.
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Coomassie brilliant blue G-250, dark reddish purple powder C.I. 42655
Supplier: MP Biomedicals
Coomassie Brilliant Blue G-250 is a protein stain in electrophoresis. Brilliant Blue G has been used in the Bradford dye-binding protein assay.
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Kits: cholesterol diagnostics, Ready-to-Load™
Supplier: EDVOTEK
Genetic testing can be used to identify people with a genetic condition which caused them to have an elevated level of cholesterol and which can be fatal. Students can see how genetic testing is carried out and learn about DNA electrophoresis.
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3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) ≥98%, yellow powder cell culture reagent
Supplier: MP Biomedicals
Storage: +4°C, protect from light
3- (4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
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Refrigerated shaking incubators, multi-functional, Incubator-Genie™ with accessories
Supplier: SCIENTIFIC INDUSTRIES ELMIS
Allows to heat, rotate, rock, and shake all in one compact unit.
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2,3,5-Triphenyltetrazolium chloride (TTC), white powder
Supplier: MP Biomedicals
2,3,5-Triphenyltetrazolium Chloride is a redox indicator. In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains as white TTC in areas of necrosis since these enzymes have been either denatured or degraded.
2,3,5-Triphenyltetrazolium Chloride is commonly used in biochemical experiments especially to indicate cellular respiration. TTC has been employed in autopsy pathology to assist identification of post-mortem myocardial infarctions. Healthy viable heart muscle will stain deep red from the cardiac lactate dehydrogenase; while areas of potential infarctions will be more pale. Useful indicator for reducing substances
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Hotplate
Supplier: Thermo Fisher Scientific
External-controlled hotplates with porcelain-coated stainless-steel top.
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Agarose multipurpose, white powder for molecular biology
Supplier: MP Biomedicals
Agarose is a natural polysaccharide isolated from agar or agar-bearing marine algae. Its physical and chemical properties are particularly suited for use as a gel medium for diffusion and electrokinetic movement of biomolecules. Agarose is essentially biologically inert and forms relatively clear, transparent gels. Agarose based gels offer several advantages: High gel strength permits use of low concentrations. The macroporous nature of the gels allows rapid diffusion of high molecular weight molecules. Agarose is Non-toxic. Agarose gels are thermally reversible facilitating sample recovery. Staining and destaining may be done rapidly with minimal background. Because agarose forms macroporous gels that are electrically nonionic, it is an excellent medium for Isoelectric Focusing (IEF). A selection of different formulated agaroses with specific qualities for unique applications are available from MP.
Multipurpose Agarose is a versatile high gel strength agarose, especially designed for a wide range of molecular biology techniques. It is used for resolution of fragments over a large size range from 200 bp up to 50 kb by conventional electrophoresis. It is suitable for use in pulsed field gel electrophoresis and for blotting assays.
High gel strength agarose, especially designed for a wide range DNA. Versatile – separate fragments from 200 bp up to 50 kb by conventional electrophoresis. Concentrations between 0.4% and 6%. Multipurpose Agarose features the highest gel strength available, allowing 0.4 - 0.5% agarose gels. Significantly decreases electrophoresis times. Improves separation of very large fragments. Dissolves easily in a microwave.
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Coomassie® brilliant blue R-250, dark powder for electrophoresis
Supplier: MP Biomedicals
Coomassie® Brilliant Blue R-250 is a fast acting, sensitive dye, which can be used on SDS gels, IEF gels, and standard gels.