"Staining Solutions"

Supplier: MP Biomedicals

The EtBr Greenbag™ Disposal Kit allows rapid and trouble-free concentration of ethdium bromide from large volumes of solutions into a small 'teabag' containing activated carbon. The Greenbag™ is then conveniently disposed of along with other solid hazardous wastes.

Supplier: VWR Chemicals

Acidified alcoholic formalin for the fixation of glycogen.

Supplier: Avantor

Microscopy and staining reagents, J.T.Baker®, Cervix spray fixative

Supplier: Avantor

Formalin (dissolved formaldehyde) is used in histopathology laboratories.

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: VWR Collection

Immersion oil is used to increase microscope resolution.

Supplier: VWR Chemicals

Q Path® Safesolv is a non hazardous replacement for xylene or toluene. It is compatible with the Q Path® Safemount mounting medium reference 00647520. It is used in place of xylene, toluene, methylecyclohexane or other solvents in the course of the dehydration stages of histological specimens and during the stages of staining and lamella mounting.

Supplier: VWR Chemicals

High viscosity mounting medium for cryotomy.

Supplier: VWR Chemicals

DC1 slow acting decalcifiers contain a mixture of formic acid and formalin.

Supplier: MP Biomedicals

Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. A 10mg/mL easy-to-use solution of ethidium bromide in specially filtered, deionized water which is excellent for nucleic acid electrophoresis and purification applications, eliminates the dust hazard associated with powdered ethidium bromide and saves time spent on weighing and mixing. Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands. Biochem/physiol Actions: Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Supplier: VWR Chemicals

Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: Honeywell Chemicals

UV-transparent, fluorescence-free immersion oil.

Supplier: VWR Chemicals

HistoChoice® clearing agent is a much less hazardous replacement for xylene/toluene.

Supplier: MP Biomedicals

Alcian Blue 8GX is a cationic dye used to quantitatively determine glycosaminoglycans by forming insoluble complexes in solution.

Supplier: VWR Chemicals

Q Path Labofix is a spray cytology fixative able to fix and store the cellular elements from gynaecological and non gynaecological smears after spreading them out on a glass slide.