3424 Results for: "NMR Spectrometry Reagents"

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® blood DNA kit provides rapid total DNA isolation from up to 250 µl of fresh or frozen anticoagulated whole blood. The E.Z.N.A.® blood DNA kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® blood DNA method is ready for applications such as PCR, southern blotting, or restriction enzyme digestion.

Supplier: OMEGA BIO-TEK

Isolate total RNA from cells or soft tissues using spin column.

Supplier: OMEGA BIO-TEK

MicroElute® Clean Up system, designed for rapid DNA clean up.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and invitro translation.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Water DNA kit is formulated to isolate high purity cellular DNA from water samples typically containing humic acid and inhibitors of PCR. This kit has been successfully used to isolate DNA from tough-to-lyse bacteria, fungi, and algae that inhabit a range of samples including tap water, lake, river and sewage samples. Isolated DNA can be used for most downstream applications including PCR, Southern blotting, SNP analysis.

Supplier: OMEGA BIO-TEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Total RNA kit II is designed for isolating total cellular RNA from tissues rich fibrous and fatty tissues such as skeletal muscle, heart, brain and adipose tissues. Compared to other standard silica-column procedures, the E.Z.N.A.® Total RNA kit II provides higher yield and better quality of RNA from all types of tissue. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue sample. RNA purified using the E.Z.N.A.® Total RNA method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Supplier: OMEGA BIO-TEK

The MicroElute RNA clean-up kit is designed to purify and concentrate RNA (from 50 μg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications require highly concentrated RNA sample.

Supplier: OMEGA BIO-TEK

Selected protease digestion releases microgram amounts of DNA from FFPE samples.

Supplier: OMEGA BIO-TEK

Isolate DNA from molluscs and insects using spin columns.

Supplier: VWR Chemicals

Both anolyte and catholyte solutions are with improved formulation to increase the speed and accuracy of titration when determining water content at the microgram level.

Supplier: Avantor

Formalin (dissolved formaldehyde) is used in histopathology laboratories.

Supplier: Biotium

EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

Supplier: Corning

These polypropylene reagent vessels are appropriate for automated and manual applications

Supplier: VWR Chemicals

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.

Supplier: VWR Collection

Immersion oil is used to increase microscope resolution.

Supplier: Avantor

Dithiothreitol (DTT, Cleland's reagent) ≥99.5%, Ultrapure Bioreagent, J.T.Baker®

Supplier: VWR Chemicals

Acidified alcoholic formalin for the fixation of glycogen.

Supplier: Thermo Fisher Scientific

Applications: Complete and ready to use reagent for the isolation of genomic DNA from solid and liquid samples of animal and plant origin

Supplier: VWR Chemicals

Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.

Supplier: VWR Chemicals

Dithiothreitol (DTT, Cleland's reagent) ≥98%

Supplier: VWR Chemicals

Anolyte for coulometric Karl Fischer titration of ketones and Li-ion battery electrolytes, preferred for cells with diaphragm, acetonitrile-based, free of alcohols (free of CMR substances)

Supplier: VWR Chemicals

These reagents are ideal for water determination including the analysis of aldehydes and ketones.

Supplier: VWR Chemicals

When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.

Supplier: Quantabio

The sparQ DNA Frag and Library Prep kit optimises the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimising DNA over fragmentation while greatly simplifying library.

Supplier: VWR Chemicals

Dithiothreitol (DTT, Cleland's reagent) ≥99.4%, Proteomics Grade

Supplier: VWR Chemicals

Catholyte for coulometric Karl Fischer titration of ketones and Li-ion battery electrolytes, Acetonitrile-based, free of alcohols (free of CMR substances).

Supplier: VWR Chemicals

2,2% ninhydrin with 0,09% tin II chloride in aqueous solution.

Supplier: VWR Chemicals

Deoxyribonucleotides are the foundation of DNA and it takes thousands of these nucleotides to make up DNA.

Supplier: VWR Chemicals

The volumetric and coulometric reagents are buffered systems, stabilised around the ideal value between 5 to 7 ensuring a rapid and stoichiometric course of reaction. Buffering systems which neutralise and stabilise the pH during the reaction are suitable for this purpose.