3440 Results for: "NMR Spectrometry Reagents"

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SATZ WIPER PROFILE 1MM 1 * 5 items

Supplier: MP Biomedicals

From Chicken Egg white Type VI. Molecular Biology Reagent3X Crystallized Salt-Free, Albumin-Free Lyophilized. Activity: =2 3,500 Shugar units/mgUnit Definition: One unit will produce a decrease in A450 of 0.001 per minute at pH 6.24 and 25C using Mi 1 * 25 g

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SYRINGE GASTIGHT 1001RNCP-1ML 1 * 1 items

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Set grad Glass R-4 An. 3ml (4) 1 * 4 items

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Jumpsuits w/o Hood (APP) with conductive knitted cuff Fabric: Grid (MR) Colour: White Size: 5XL 1 * 1 items

Supplier: MP Biomedicals

Storage: Store at -20 °C.
Brefeldin A is a fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. Produced by Penicillium brefeldianum. Blocks binding of the cytosolic coat protein b-COP and ARF to Golgi membranes mediated by protein G. Also blocks protein transportation into post-Golgi compartments. It activates the sphingomyelin cycle. Brefeldin A mediated apoptosis has been observed in human tumor cells.
Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles.
Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells.

Supplier: MP Biomedicals

White solid, soluble in water or aqueous buffers (50 mg/ml); soluble in 0,01 N Sodium hydroxide (100 mg/ml - clear to slightly hazy, yellowish solution).

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PLUG REPLACEMENT ONLY 2MM 1 * 1 items

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Jumpsuits w/o Hood (APP) with conductive knitted cuff Fabric: Grid (MR) Colour: White Size: 6XL 1 * 1 items

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48R Control PCB high temperature Gen 2 48R Control PCB high tempe- rature Gen 2 1 * 1 items

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GRADUATED CYLINDER 50ML 1ML ±0.25ML 1 * 1 items

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Set grad.glass R-6 RV 1ml,6pc 1 * 6 items

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2ML VIAL FREEZER RACK 552X141X240MM 1 * 1 items

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CALCIUM CHLORIDE 2MG/L 946 ML 1 * 1 items

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O-RING ID 11MM X 3MM EPDM 1 * 1 items

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Nozzle,3mm,316 ss,z61 1 * 1 items

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SLIDE IMMUNO 100X200MM +/-1MM 1 * 1.000 items

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HOSE PVC 10MM THICKN 2MM 1 * 100 m

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FLASK CMPLT 1ML,CERTIFIED BY LOT 1 * 1 items

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GMTR SYNC 230V50HZ/40R RHS6A6C 1 * 1 items

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48R Control PCB non high temperat. Gen2 48R Control PCB non high temperature Gen 2 1 * 1 items

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25x Ferrules D:2mm, black 1 * 25 items

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GLASS BALLS 3MM BOROSILICATE 1 * 1 items

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MCE: FOMIVIRSEN SODIUM , 5MG 1 * 5 mg

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

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Set of 25 plugs 3mm,25pcs 1 * 25 items