2493 Results for: "Epigenetics Reagents"

Supplier: Trajan Scientific and Medical

eVol® is the coupling of two precision devices: A digitally-controlled electronic drive and an XCHANGE®-enabled analytical syringe. The result is a digitally-controlled, positive-displacement dispensing system that can be programmed to reproducibly and accurately perform a wide variety of liquid handling procedures.

Supplier: EPPENDORF

Eppendorf epT.I.P.S.® Racks feature high-precision pipette tips in 'Eppendorf Quality', 'PCR clean', 'Biopur®', and 'Sterile' purity grades. All tips are supplied in disposible racks designed for optimal organisation and user convenience in demanding laboratory environments.

Supplier: EPPENDORF

Eppendorf epT.I.P.S.® Bulk packs offer a practical, high-volume solution with 'Eppendorf Quality' pipette tips, ensuring precise and reliable measurements for various lab applications, packaged economically for high-throughput settings.

Supplier: MP Biomedicals

Brilliant blue G is a useful dye for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualised during staining. It has been used in the Bradford dye-binding protein assay.

Supplier: MP Biomedicals

Protamine sulphate is a purified mixture of simple protein principles obtained from the sperm or testes of suitable species of fish, which has the property of neutralizing heparin. Because of having much basic amino acids (mostly arginine) protamine contains far greater nitrogen than other proteins. Its molecular weight is relatively small. Histone and other basic proteins in the testes of unmatured fishes convert into protamine along with the growth of the fishes. In the testes protamine takes the form of nucleoprotamine linked with DNA.

Supplier: MP Biomedicals

White Powder, soluble in water or aqueous buffers (50 mg/ml); soluble in 0,01 N Sodium hydroxide (100 mg/ml - clear to slightly hazy, yellowish solution).

Supplier: MP Biomedicals

Storage: Room Temperature
Guanidine hydrochloride consists of a network of guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds, it is a strong chaotropic agent. Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a free flowing powder with solid material dispersed throughout, or a solid. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine HCl appear identical.
Guanidine hydrochloride can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA. Guanidine hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of protein, it can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins.

Supplier: MP Biomedicals

Sodium chloride is a commonly used chemical which is found widely in nature. It is considered to be an essential nutrient. Excess amounts of sodium chloride can destroy electrolyte balance and cause death in most animals, including humans.
Sodium chloride is used in a wide variety of biochemical applications, including intravenous fluids (normal saline is 0.9% w/v in water 10), density gradients 3,6, a diluent to increase ionic strength in buffers or culture media and in salt-out procedures in the isolation of DNA. It has also been used in high concentrations for preservation of foods since bacteria cannot grow in high salt conditions. A salt-ice mixture in the ratio of approximately 33 g sodium chloride to 100 g ice (at -1°C) will drop in temperature to as low as -21°C, depending on the rate of stirring and the size of the ice chunks.

Supplier: Corning

PP, graduated. The DeckWorks™ system is designed to maximise storage space and help reduce the production and waste of plastic materials. Both the hinged racks and reload decks within this system are manufactured with recycled plastic.

Supplier: HEATHROW

Compact digital orbital shaker which is packed full of high end design features making this laboratory shaker a versatile unit for a vast range of protocols.

Supplier: MP Biomedicals

Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Supplier: MP Biomedicals

Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc.
Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture selective protein extraction growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
Soluble/miscible in water to give a clear yellow solution; miscible with alcohol, dioxane, and ethyl acetate; practically insoluble in liquid paraffin and fixed oils (such as mineral oil).
Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter.
Store at Room Temperature (15-30 °C).

Supplier: MP Biomedicals

Applications: Ready-Red™ is used either to remove traces of phenol from the aqueous phase or can be mixed directly with AquaPhenol™ (void volume in AquaPhenol™ bottle is adapted to this purpose).
Product Overview: Ready-Red™ is a Chloroform:isoamyl alcohol 24:1 with red dye solution which facilitates separation of organic and aqueous phases. It improves recovery.
Product Description: Ready-Red™ is a coloured mixture of chloroform and isoamyl alcohol. The red colour (non toxic dye), facilitates the visualization and the separation of aqueous and organic phases during nucleic acids purification by means of phenolic extractions. The red dye of Ready-Red™ is not water-soluble and does not interfere with the quality of the nucleic acids purified. The nucleic acids recovered can be used in all standard experiments of molecular biology such as sequencing, PCR, cloning, transformations, etc.

Supplier: VWR Collection

This compact system produces EDI-based Type II water directly from tap water. Quality of Type II water meets or exceeds water quality as defined by ASTM, CAP, CLSI and ISO 3696 / BS 3997, and also complies with the purified water requirements from the European and U.S. Pharmacopoeia.

Supplier: MP Biomedicals

White solid, soluble in water or aqueous buffers (50 mg/ml); soluble in 0,01 N Sodium hydroxide (100 mg/ml - clear to slightly hazy, yellowish solution).

Supplier: MP Biomedicals

Storage: Store at -20 °C.
Brefeldin A is a fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. Produced by Penicillium brefeldianum. Blocks binding of the cytosolic coat protein b-COP and ARF to Golgi membranes mediated by protein G. Also blocks protein transportation into post-Golgi compartments. It activates the sphingomyelin cycle. Brefeldin A mediated apoptosis has been observed in human tumor cells.
Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles.
Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: MP Biomedicals

Potassium sodium tartrate tetrahydrate has been used in organic synthesis to break up emulsions in aqueous workups.

Supplier: Honeywell Chemicals

MAGNESIUM SULFATE, ANHYDROUS, REAGENTPL& 1 * 2 kg

Supplier: Honeywell Chemicals

METAPHOSPHORIC ACID, CHIP, A.C.S. REAGEN T 1 * 5 g

Supplier: Honeywell Chemicals

METAPHOSPHORIC ACID, CHIP, A.C.S. REAGEN T 1 * 100 g

Supplier: MP Biomedicals

AGAROSE. Molecular Biology ReagentIdeal for nucleic acid electrophoresis, analysis and purification.EEO: 0.09-0.13Moisture: No detec table DNase, RNase, or protease. 1 * 10 g

Supplier: MP Biomedicals

AGAROSE. Molecular Biology ReagentIdeal for nucleic acid electrophoresis, analysis and purification.EEO: 0.09-0.13Moisture: No detec table DNase, RNase, or protease. 1 * 100 g

Supplier: MP Biomedicals

AGAROSE. Molecular Biology ReagentIdeal for nucleic acid electrophoresis, analysis and purification.EEO: 0.09-0.13Moisture: No detec table DNase, RNase, or protease. 1 * 25 g

Supplier: MP Biomedicals

AGAROSE. Molecular Biology ReagentIdeal for nucleic acid electrophoresis, analysis and purification.EEO: 0.09-0.13Moisture: No detec table DNase, RNase, or protease. 1 * 500 g

Supplier: MP Biomedicals

AGAROSE. Molecular Biology ReagentIdeal for nucleic acid electrophoresis, analysis and purification.EEO: 0.09-0.13Moisture: No detec table DNase, RNase, or protease. 1 * 50 g

Supplier: MP Biomedicals

YEAST EXTRACT. Molecular Biology ReagentA vacuum-dried extract concentrate of Baker's yeast containing the B-complex factors of appr oximately three times the weight of ordinary dry yeast. An alternative to beef extract for general bacteriological us 1 * 250 g

Supplier: MP Biomedicals

YEAST EXTRACT. Molecular Biology ReagentA vacuum-dried extract concentrate of Baker's yeast containing the B-complex factors of appr oximately three times the weight of ordinary dry yeast. An alternative to beef extract for general bacteriological us 1 * 1 kg

Supplier: MP Biomedicals

METHYLENE BLUE TRIHYDRATE. Zinc FreeTrihydrateMolecular Biology Reagent.An ethidium bromide alternative for the visualization of nucleic acids in gels.Gels are viewable in visible light.Does not require a darkroom.No UV induced DNA damage Gels can be 1 * 5 g

Supplier: MP Biomedicals

METHYLENE BLUE TRIHYDRATE. Zinc FreeTrihydrateMolecular Biology Reagent.An ethidium bromide alternative for the visualization of nucleic acids in gels.Gels are viewable in visible light.Does not require a darkroom.No UV induced DNA damage Gels can be 1 * 100 g