141 Results for: "Electrophoresis Buffers"

Supplier: PanReac AppliChem

50X concentrated solution of 2 M Tris, 1 M glacial acetic acid and 0,5 M EDTA.Na₂, pH 8,5 ±0,2.

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

Supplier: PanReac AppliChem

10X concentrated solution of 40 mM Tris, 20 mM glacial acetic acid and 1 mM EDTA.Na₂, pH 8,5 ±0,2.

Supplier: MP Biomedicals

Electrophoresis Grade Heavy metals (as Pb): 20 ppm Used for preparation of Tris-glycine buffers.

Supplier: PAN BIOTECH

Running Buffer, TAE buffer, 50X

Supplier: MP Biomedicals

Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.

Supplier: VWR Collection

Designed for ease of use and safe operation, the shiroGEL horizontal electrophoresis systems and accessories are built to withstand the rigours of everyday use. For leakproof performance the gel boxes and gel trays are moulded from thick acrylic. UV transparent gel trays aid in visualisation of samples. Cassettes protect the electrodes and allow for easy replacement. Gel casting is simple with the durable rubber casting gates. Slots in the sides of the trays allow for easy comb placement. The two-piece design of the combs allows for vertical adjustment, giving the user control over the depth of the well. The combs supplied with the systems are 1,5 mm thick. Multiple combs, including those compatible with multichannel pipettes and different thicknesses, are also available. The 'EasyLift' gel box lid is easily removed using the side tabs and pressure pads. The lid is domed, to prevent condensation from dripping onto the gel, and is surrounded by a drip ring, to help recover the condensate and maintain buffer concentration.

Supplier: MP Biomedicals

Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.

Supplier: MP Biomedicals

Sodium acetate is used as a buffer in the buffering range of pH 3.6-5.6. It is also used in the purification and precipitation of nucleic acids, protein crystallization, staining of gels in protein gel electrophoresis, HPLC, mordant dyeing, DNA microarray studies of E. coli response, investigation of protein unfolding during reverse phase chromatography.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Supplier: MP Biomedicals

Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15 - 30 °C)
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. Glycerol is used in the concentration and storage of enzymes. Also prevents back-diffusion and protein samples into the buffer. Ideal for use as a cryoprotectant.

Supplier: MP Biomedicals

Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). Tris does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is an auxiliary material in pharmaceutical science.
Store at Room Temperature (15-30 °C). Store dessicated.

Supplier: MP Biomedicals

Borax is a salt of boric acid which is used as a buffer. It has a co-complexing ability with other agents in water to form complex ions with various substances. It is also commonly utilized in chromatography and electrophoresis studies.

Supplier: MP Biomedicals

Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.

Supplier: MP Biomedicals

Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is RNAse and DNAse-free. Tris is relatively non-hygroscopic ; but, if needed, it can be dried at 100 °C for up to 4 hours to remove any water.
Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.

Supplier: MP Biomedicals

This product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. Specificity for human IgG is determined by immunoelectrophoresis (IEP) versus purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins.Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation in the gamma region.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: MP Biomedicals

DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.

Supplier: G-Biosciences

molecular grade, concentrated running buffer for dna and rna electrophoresis. 1 * 250 mL

Supplier: VWR Collection

VWR® Real Time electrophoresis systems have revolutionised DNA electrophoresis. it is no longer necessary to remove a gel from a tank, place it onto a transilluminator for viewing or place the gel into an imaging system to take a photo. These versatile and compact systems are an all-in-one replacement for a power supply, gel tank, transilluminator and gel imaging system.

Supplier: CLEAVER SCIENTIFIC

omniPAGE mini wide vertical gel unit with an active gel width of 18 cm effectively allows double the number of samples to be resolved as the omniPAGE mini unit. This allows consistency of sample comparison on a single gel and is designed for those with greater than 20 samples to compare and resolve.

Supplier: VWR Chemicals

VWR® DNA ladders are convenient dsDNA ladders supplied in a loading buffer, which are ready to use on agarose and SDS DNA gels.

Supplier: VWR Collection

The use of levelling platforms in electrophoresis can be a big help in maintaining a uniform gel thickness and therefore a uniform and quality sample separation.

Supplier: G-Biosciences

A single step stain for the rapid detection of proteins resolved by PAGE. The stain is based on the interaction of zinc ions with polyacrylamide and proteins.

Supplier: Corning

Axygen® horizontal gel box is a high quality, full-featured unit designed for exceptional durability and efficiency. These gel boxes feature leak-free external gel casting, which allows the user to cast one gel outside of the box while another gel is running inside. Boxes from 7, 10, 15 and 20cm

Supplier: MP Biomedicals

Soluble in water (50 g/ml).

Supplier: PanReac AppliChem

DNA-Dye NonTox is a highly sensitive, non toxic, fluorescent staining reagent for the detection of double-stranded DNA (dsDNA). The dye produces instant visualisation of DNA bands on gels upon blue light or UV illumination.

Supplier: MP Biomedicals

Soluble in water or dilute buffer.

Supplier: PanReac AppliChem

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.3% suitable for buffer solutions