One-Step SYBR® Green qRT-PCR, qScript™
qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.
: Kits are available with or without ROX or with fluorescein for Bio-Rad iCycler iQ® systems. All kits contain qScript One-Step Reverse Transcriptase (optimised 50X formulation of recombinant MMLV reverse transcriptase for one-step RT-PCR); One-Step SYBR Green Master Mix (2X) reaction buffer containing dNTPs, MgCl₂, AccuStart™ Taq DNA polymerase, stabilisers, and SYBR® Green I dye; and nuclease-free water.
- Application:RT-qPCR dye
- One step:Yes