Specifications
- Antibody type:Primary
- Antigen name:beta-Galactosidase
- Antigen symbol:b-Gal
- Clonality:Polyclonal
- Host:Goat
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Isotype:IgG
- Reactivity:E. coli
- Western blot:Yes
- Environmentally Preferable:
- Form:Liquid
- Gene ID:945006 Escherichia coli
- Storage temperature:+2 °C to +8 °C
- Concentration:Total Protein: 31.1 mg/mL
- Shipping temperature:Cold Pack
- Immunogen:Recombinant full length protein of Escherichia coli β Galactosidase
- Tested applications:Cell or tissue immunostaining
- Purification:Delipidation, ammonium sulfate fractionation, and anion exchange chromatography followed by solid-phase absorption
- Size:1 ml
- Pk:1 mL
Specifications
About this item
Goat IgG fraction to beta-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques.
The antibody is produced by hyperimmunizing goats with the enzyme beta-galactosidase from Escherichia coli. The IgG fraction is prepared by delipidation, ammonium sulfate fractionation, and anion exchange chromatography, and is further purified by solid-phase absorption to remove antibodies to non-beta-galactosidase E. coli proteins. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed. No preservative is added.
- Dilution Range:1:50 - 1:200 for Tissue Staining
- Dilution Range:1:100 - 1:500 for Immunoblotting
- Dilution Range:1:400 - 2,000 for ELISA
Suitable for conjugation with enzymes, radiolabels, fluorochromes, or other markers; suitable for attachment to solid supports for use as an immunoadsorbant. It is specially prepared for the detection and purification of recombinant proteins.
Type: Primary
Antigen: IGG fraction to beta-galactosidase
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: Goat
Isotype:
Reactivity: E.coli