Actin Monoclonal Antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.It is also used in indirect ELISA, immunofluorescence, immunoblots and immunohistochemistry

• Dilution range:1:500 for indirect immunofluorescence
• Dilution range:1:500 for immunoblots (for DAB/peroxidase detection, actin band migrates at 42 kDa on SDS-PAGE)
• Dilution range:1:500 for immunohistochemistry

Antibodies to cytoskeletal proteins are widely used for protein loading controls or specific studies such as apoptosis.It detects all actins or tubulins.It is presented as filtered ascites containing 0,01% sodium azide as a preservative.

Type: Primary
Antigen: Actin
Clonality: Monoclonal
Clone: C4
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Mammals, yeast, fungi