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Nucleic Acid Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

Plasmid purification, MiniPrep Express™ Matrix, MP Biomedicals

Plasmid purification, MiniPrep Express™ Matrix, MP Biomedicals

Supplier: MP Biomedicals

MiniPrep Express™ Matrix is designed for rapid purification of plasmid DNA. It is an ideal tool for screening hundreds of recombinant clones in record time using restriction enzyme analysis and PCR.

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DNA purification kits, GeneClean® for Ancient DNA™ Kit, MP Biomedicals

DNA purification kits, GeneClean® for Ancient DNA™ Kit, MP Biomedicals

Supplier: MP Biomedicals

The GeneClean® for Ancient DNA™ Kit is ideal for purification of fragmented or damaged DNA from aged or preserved samples. It is designed for isolation of up to 20 μg DNA from bone, preserved tissue, soil organisms, or animal by-products.

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DNA extraction kits, tissue DNA extraction kit, MPure™, MP Biomedicals

DNA extraction kits, tissue DNA extraction kit, MPure™, MP Biomedicals

Supplier: MP Biomedicals

MPure™ Tissue DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction and purification of genomic DNA from a variety of animal tissues, swab samples and blood stains. Nucleic acids extracted and purified from MPure™ Tissue DNA Extraction Kits can be used in a number of downstream applications including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP and Southern Blot Analysis.

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DNA extraction kits, MPure™ Bacterial DNA extraction kit, MP Biomedicals

DNA extraction kits, MPure™ Bacterial DNA extraction kit, MP Biomedicals

Supplier: MP Biomedicals

MPure™ Bacterial DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction and purification of genomic DNA from both gram-positive and gram-negative bacteria. Nucleic acids extracted and purified from MPure™ Bacterial DNA Extraction Kit can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP and Southern Blot Analysis.

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DNA clean up kits, GeneClean® Turbo Kits, MP Biomedicals

DNA clean up kits, GeneClean® Turbo Kits, MP Biomedicals

Supplier: MP Biomedicals

GeneClean® Turbo Kit is used for purification of DNA fragments of sizes 0,1 kb to 300 kb from TAE or TBE buffered agarose gels, PCR reactions and other enzymatic solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

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Genomic DNA isolation from tissues, NucleoSpin® 8/96 tissues/Tissue Core kits

Genomic DNA isolation from tissues, NucleoSpin® 8/96 tissues/Tissue Core kits

Supplier: MACHEREY-NAGEL

NucleoSpin® 8/96 Tissue and NucleoSpin® 8/96 Tissue Core kits for isolation of genomic DNA from human or animal tissue, cultured cells, or bacteria. Available with a choice of flexible 8-well strip format for varying throughput or 96-well format for high throughput.

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Spin module and recovery tubes for DNA purification, MP Biomedicals

Supplier: MP Biomedicals

Spin module and recovery tubes are used to separate particles from solutions in a few seconds. They are designed for optimal performance, providing faster and cleaner separations than traditional methods, such as columns, filters, or simple centrifugation.

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RNA extraction, MPure™ Total RNA extraction kit , MP Biomedicals

Supplier: MP Biomedicals

MPure™ Total RNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction of total RNA from whole blood, blood cells, animal tissue, plant tissue, yeast or cultured cells and viral RNA from human biological specimens such as serum, plasma, and other cell-free fluids.

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DNA extraction, MPure™ Blood DNA extraction kits, MP Biomedicals

DNA extraction, MPure™ Blood DNA extraction kits, MP Biomedicals

Supplier: MP Biomedicals

MPure™ Blood DNA Extraction Kit is used with the MPure-12™ aNAP System for extraction of DNA from 100 to 400 µl mammalian whole blood, peripheral blood mononuclear cells or buffy coat. Nucleic acids extracted from MPure Blood DNA Extraction Kit 200 can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis.

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DNA purification kits, GeneClean® II kit, MP Biomedicals

DNA purification kits, GeneClean® II kit, MP Biomedicals

Supplier: MP Biomedicals

The GeneClean® II Kit is designed to optimally purify 200 bp to 20 kb DNA from TAE and TBE buffered agarose gels and solutions. The purified DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.

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Power supplies, Axygen®

Supplier: Corning

Axygen power supplies are ideal for most electrophoresis needs.

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Mag plasmid kit, Axygen® AxyPrep™

Supplier: Corning

The AxyPrep™ Mag Plasmid purification kit utilises a magnetic bead technology for high-throughput purification of plasmid DNA from E.coli cells.

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AxyPrep™ FragmentSelect-I kit, Axygen®

AxyPrep™ FragmentSelect-I kit, Axygen®

Supplier: Corning

The AxyPrep™ FragmentSelect-I Kit is optimized for Illumina next generation sequencing platforms and Life Technologies (SOLiD) fragment size selection needs to simplify the library construction workflow. The kit is designed for fragment size selection needs in the Roche/454’s DNA Genome sequencer library construction workflow.

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Mag PCR normalizer kit, Axygen® AxyPrep

Mag PCR normalizer kit, Axygen® AxyPrep

Supplier: Corning

The AxyPrep™ Mag PCR Normalizer Kit utilises a paramagnetic bead-based purification system for PCR clean up.

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Genomic DNA isolation from tissue, Axygen® AxyPrep™ Mag

Genomic DNA isolation from tissue, Axygen® AxyPrep™ Mag

Supplier: Corning

The AxyPrep™ Mag Tissue gDNA Kit utilises a magnetic bead-based technology to isolate genomic DNA from rodent tail tissues. DNA from other tissue types may be extracted with this protocol, but optimisation may be required.

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TRItidy G™

TRItidy G™

Supplier: PanReac AppliChem

TRItidy G™ is based on the single step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples (Chomczynski, P. (1993), BioTechniques 15, p.532 - 537). RNA is selectively retained in the aqueous phase during acidic GuSCN/phenol extraction; DNA and proteins are isolated from the organic phase by ethanol and isopropanol precipitation, respectively.

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Axygen® AxyPrep® Mag DyeClean kit

Axygen® AxyPrep® Mag DyeClean kit

Supplier: Corning

Removing excess dye terminator is an essential step prior to Sanger sequencing. Carryover of the excess dye into the sequencing reactions may result in dye blobs resulting in inaccurate results. The AxyPrep™ Mag DyeClean kit utilises a paramagnetic bead-based purification system for Sanger sequencing reaction dye terminator clean-up. The protocol is simple and comprises binding, washing and elution steps which can be performed directly in the thermal cycling plate. This kit requires no centrifugation or filtration steps.

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Plasmid purification kit, Axygen® AxyPrep™ Mag

Plasmid purification kit, Axygen® AxyPrep™ Mag

Supplier: Corning

AxyPrep™ Mag Plasmid Purification Kit utilises a magnetic bead technology for high throughput purification of plasmid DNA from E.coli cells. The AxyPrep™ Mag kit can also be used with fosmid and BAC vector-based constructs ranging from 5 to 150 Kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralisation, DNA binding, ethanol washing and plasmid elution. The protocol is automation-compatible.

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Genomic DNA extraction kits, plant gDNA kit, Axygen® AxyPrep™ Mag

Genomic DNA extraction kits, plant gDNA kit, Axygen® AxyPrep™ Mag

Supplier: Corning

Axygen® AxyPrep™ Mag Plant gDNA Kit is a magnetic bead-based purification system that allows rapid and reliable isolation of high quality gDNA from a wide variety of plant samples. Up to 96 samples (50 mg fresh tissue per sample or 15 mg of dried tissue per sample) can be processed in less than one hour. The Axygen® AxyPrep™ Mag Plant gDNA Kit eliminates polysaccharides, phenolic compounds and enzyme inhibitors with no organic extractions. This kit is adaptable for use on major liquid handling workstations for high throughput processing.

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dNTP mix

Supplier: PAN BIOTECH

A ready-to-use molecular grade dNTP Mix containing dATP, dCTP, dGTP and dTTP at pH 7,5 as lithium salts in purified
water. The mix is designed to save hands-on time for researchers and minimise the possibility of contamination.

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sparQ universal library quant kit

Supplier: Quantabio

sparQ Universal Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms.

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PCR Master Mix, sparQ HiFi

PCR Master Mix, sparQ HiFi

Supplier: Quantabio

sparQ HiFi PCR Master Mix was specially designed for high efficiency library amplification from low DNA input. sparQ HiFi PCR Master Mix minimises the number of amplification cycles required to achieve the threshold required for sequencing. The result is a superior performing high fidelity master mix that increases library yield which reduces PCR-derived artifacts for a variety of sequencing applications.

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sparQ PureMag beads

sparQ PureMag beads

Supplier: Quantabio

sparQ PureMag beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

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DNA Library Prep Kit, sparQ

DNA Library Prep Kit, sparQ

Supplier: Quantabio

An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.

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DNA fragment and library prep kit, sparQ

DNA fragment and library prep kit, sparQ

Supplier: Quantabio

The sparQ DNA Frag and Library Prep kit optimises the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimising DNA over fragmentation while greatly simplifying library.

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Yeast transformation kit, Alkali-Cation™, MP Biomedicals

Supplier: MP Biomedicals

Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.

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Transformation kit, EZ-Yeast™, MP Biomedicals

Supplier: MP Biomedicals

The EZ-Yeast™ Transformation Kit is designed for high throughput transformation with efficiencies up to 10³ transformants per μg plasmid DNA. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. Perfect for a quick analysis of potentially positive two-hybrid colonies, it does not require fully competent cells prior to transformation.

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Cloning supplement

Supplier: PAN BIOTECH

This supplement stored at −20 °C.

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