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Electrophoresis Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

Laemmli SDS sample buffer, non-reducing (6X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, non-reducing, 6X

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Laemmli SDS sample buffer, non-reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, non-reducing, 4X

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TRIS-Tricine-SDS running buffer (10X), cathode buffer, pH 8,3

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-Tricine-SDS running buffer (10X), cathode buffer

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TBE running buffer (10X)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE running buffer (10X)

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TBS with Tween® (TBST), 20X solution, Molecular Biology Grade, ultrapure

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

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LDS-sample buffer (4X), non-reducing

Supplier: Thermo Fisher Scientific

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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TBE urea sample buffer (2X)

Supplier: Thermo Fisher Scientific

Loading Buffer, TBE Urea Sample Buffer, 2X

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TAE buffer, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.

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MOPS-SDS running buffer (20X)

Supplier: Thermo Fisher Scientific

Running Buffer, MOPS-SDS running buffer (20X)

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Laemmli SDS sample buffer, reducing (6X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 6X

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Buffers, TBS, 20X solution, pH 7,4, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Used in Western blotting protocols.

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TRIS-buffered saline (TBS, 20X) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X)

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VWR® TBS/Tween® 20X Washing Buffer, Liquid Concentrate, UltraPure Grade

VWR® TBS/Tween® 20X Washing Buffer, Liquid Concentrate, UltraPure Grade

Supplier: VWR Chemicals

Convenient liquid concentrate that produces 10 litres of 1X buffer for blotting and ELISA applications.

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VWR®RapidBlock™, Blocking solution, 10x

VWR®RapidBlock™, Blocking solution, 10x

Supplier: VWR Chemicals

RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.

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PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

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Laemmli SDS sample buffer, reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: OMEGA BIO-TEK

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Tris-Glycine (TG) buffer,, MP Biomedicals

Supplier: MP Biomedicals

Tris-glycine buffer is used for western blotting and gel electrophoresis.

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Buffer, SSC 20X powder for molecular biology, MP Biomedicals

Supplier: MP Biomedicals

SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.

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Buffer, PBS solution 10X concentrate (phosphate buffered saline), MP Biomedicals

Supplier: MP Biomedicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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SSC 20X buffer, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer), MP Biomedicals

Supplier: MP Biomedicals

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Tris-Borate-EDTA (TBE) buffer, 10X

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

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Buffer, PBS (1X, Dulbecco's) powder pack

Buffer, PBS (1X, Dulbecco's) powder pack

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (1X) according to Dulbecco.

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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution

Supplier: PanReac AppliChem

Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: PanReac AppliChem

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer) pH 8,5

Supplier: PanReac AppliChem

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer) pH 8,5

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PBS tablets

PBS tablets

Supplier: PanReac AppliChem

Tablets for preparation of 100 ml phosphate buffered saline (PBS).

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Buffer, PBS tablets

Buffer, PBS tablets

Supplier: PanReac AppliChem

PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.

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