Cell Culture Buffers
Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.
VWR® UltraPure, Buffer, PBS solution (phosphate buffered saline)
Supplier: VWR Chemicals
Dulbecco's formulation without calcium and magnesium.
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VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline)
Supplier: VWR Chemicals
Cell culture buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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VWR®, Cell culture salt solutions, Dulbecco's Phosphate Buffered Saline (DPBS)
Supplier: VWR Chemicals
DPBS is commonly used salt solution in cell enumeration as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS is also used in FACS (Fluorescence-Activated Cell Sorting) process to wash and re-suspend cells during the dissociation process, as well as for many analytical methods or biochemical treatments.
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VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology
Supplier: VWR Chemicals
Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)
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VWR®, Phosphate buffered saline (PBS) 1X, for biotechnology
Supplier: VWR Chemicals
137 mM sodium chloride, 2,7 mM potassium chloride, 12 mM phosphate buffer
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VWR® UltraPure, Phosphate buffered saline (PBS), powdered
Supplier: VWR Chemicals
PBS, Ultra Pure Grade is a powdered, magnesium- and calcium-free formulation of PBS suitable for cell culture and for use in flow cytometry applications.
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Krebs-Ringer solution, HEPES-buffered
Supplier: Thermo Fisher Scientific
Contains 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, 5,5 mM HEPES, and 1 mM D-glucose
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TE buffer, pH 8,1, RNAse free
Supplier: Thermo Fisher Scientific
Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.
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HEPES-buffered saline, pH 7,0 (2X for transfection)
Supplier: Thermo Fisher Scientific
Cell culture buffer, HEPES-buffered Saline
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Phosphate buffered saline, PBS-TWEEN® tablets, Millipore®
Supplier: MERCK ONCOGENE
Developed for use in immunoassay applications.
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Hank's balanced salt solution, modified, MP Biomedicals
Supplier: MP Biomedicals
Cell culture buffer, Hank's balanced salt solution, modified (1X)
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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium
Supplier: MP Biomedicals
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)
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STERI-BUFFER
Supplier: VELTEK ASSOCIATES
Developed for use as a buffer solution and diluent in lab testing such a bioburden testing, pharmaceutical microbial limits testing, and AET testing and as a rinsing agent.
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Hank's balanced salts, MP Biomedicals
Supplier: MP Biomedicals
Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals
Supplier: MP Biomedicals
Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.
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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals
Supplier: MP Biomedicals
Used to minimise cell attachment or aggregation.