You searched for: Urea
Urea 98.0-102.0% USP, Multi-Compendial, J.T.Baker®
Supplier: Avantor
Urea 98.0-102.0% USP, Multi-Compendial, J.T.Baker®
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Urea 98.0-102.0% USP, J.T.Baker®
Supplier: Avantor
Urea 98.0-102.0% USP, J.T.Baker®
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Urea ≥99.5%, high purity
Supplier: VWR Chemicals
Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins.
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Urea 98.0-102% USP, Macron Fine Chemicals™
Supplier: MACRON AVANTOR BRAND
Urea 98.0-102% USP, Macron Fine Chemicals™
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Urea 8 mol/l in aqueous solution, Proteomics Grade
Supplier: VWR Chemicals
A convenient pre-mixed solution that is easily diluted for buffer preparation under denaturing conditions.Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions.
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Urea ≥99.5%, Ultrapure Bioreagent, J.T.Baker®
Supplier: Avantor
For protein solubilization and denaturation. DNase-, RNase-, protease-, and cyanate-free.
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Urea 98.0-102.0% USP, J.T.Baker®
Supplier: Avantor
Urea 98.0-102.0% USP, J.T.Baker®
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Urea 99.0-100.5%, BAKER ANALYZED® ACS, J.T.Baker®
Supplier: Avantor
Urea 99.0-100.5%, BAKER ANALYZED® ACS, J.T.Baker®
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Urea 99.0-100.5%, crystals, AR® ACS, Macron Fine Chemicals™
Supplier: MACRON AVANTOR BRAND
Urea 99.0-100.5%, crystals, AR® ACS, Macron Fine Chemicals™
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Urea ≥99.5%, Proteomics Grade
Supplier: VWR Chemicals
Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols.
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Urea 99.5-100.5%, AnalaR NORMAPUR® Reag. Ph. Eur., ACS analytical reagent
Supplier: VWR Chemicals
Urea 99.5-100.5%, AnalaR NORMAPUR® Reag. Ph. Eur., ACS analytical reagent
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Urea ≥99.5%, Ultrapure for molecular biology
Supplier: Thermo Fisher Scientific
Urea ≥99.5%, Ultrapure for molecular biology
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Urea 99.0-100.5% ACS
Supplier: Thermo Fisher Scientific
Urea 99.0-100.5% ACS
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Urea ≥99%, white prills, Ultrapure
Supplier: MP Biomedicals
Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
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Urea ≥99%, white crystalline powder
Supplier: MP Biomedicals
Urea is a mild agent usually used in the solubilization and denaturation of proteins.
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Urea, white prills, Reagent Grade
Supplier: MP Biomedicals
Urea is a prinicipal protein metabolite end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle and the major product for the removal of free ammonia (NH4+) in vivo.
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Urea ≥98%, white powder for molecular biology
Supplier: MP Biomedicals
Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C
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Urea ≥99%, white powder, Ultrapure
Supplier: MP Biomedicals
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex.