Enzymes
Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.
VWR®, HL-dsDNase
Supplier: VWR Chemicals
VWR® Life Science's DNase, Double-Strand Specific, Heat-Labile is a recombinant endonuclease that cleaves phosphodiester bonds in DNA to yield 2 to 8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini.
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VWR®, Aspergillus Glucose oxidase
Supplier: VWR Chemicals
Beta-D-glucose oxidase with high purity grade and storage in frozen condition.
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J.T.Baker® Endonuclease
Supplier: Avantor
J.T.Baker® Endonuclease Biotech Reagent meets the strictest cGMP standards and is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Biotech Reagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease Biotech Reagent is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. Absence of proteolytic activity. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Biotech Reagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.
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VWR®, Cod Uracil-DNA glycosylase
Supplier: VWR Chemicals
Uracil-DNA glycosylase (UNG), cod is a thermolabile recombinant enzyme produced in E. coli (ung-) using a modified ung gene derived from Atlantic cod. It degrades uracil-containing single- and double-stranded DNA, but not RNA or thymidine-containing DNA, by hydrolysing the N-glycosidic bond between deoxyribose sugar and the base in uracil. This generates alkaline-sensitive apyramidinic sites in the DNA that will be cleaved upon a combination of alkaline conditions and high temperature.
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VWR®, Bovine deoxyribonuclease I (from Pancreas)
Supplier: VWR Chemicals
Deoxyribonuclease I efficiently hydrolyses single-stranded or double-stranded DNA in the presence of divalent cations. Useful in RNA preparations from tissue or bacterial cell cultures, DNase I is a chromatographically purified preparation supplied as a lyophilised powder.
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J.T.Baker® Endonuclease
Supplier: Avantor
J.T.Baker® Endonuclease Ultrapure Bioreagent is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Ultrapure Bioreagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Ultrapure Bioreagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.
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VWR®, Pronase E
Supplier: VWR Chemicals
Off-white to light brown powder from Streptomyces griseus.
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VWR® Trichoderma viride Cellulase
Supplier: VWR Chemicals
10,000 units is that quantity of enzyme which will degrade 2 cm² of cellulose filter paper (17 mg) in 1 minute in 0,1 M acetate buffer at pH 4,0.
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Elastase substrate V
Supplier: Thermo Fisher Scientific
Elastase substrate V
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RNase A
Supplier: OMEGA BIO-TEK
Ribonuclease A (RNase A) is endonuclease-free and quality controlled for use in plasmid purification procedures for digestion of RNA.
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S5-Alcohol Dehydrogenase
Supplier: Thermo Fisher Scientific
Enzymic catalysis in organic solvents.
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Pig Desoxyribonuclease II (from Spleen)
Supplier: Thermo Fisher Scientific
Pig Desoxyribonuclease II (from Spleen)
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Alcohol dehydrogenase
Supplier: Thermo Fisher Scientific
Alcohol dehydrogenase catalyses the reaction: RCH₂OH +NAD⁺ → RCHO + NADH + H⁺. It facilitates the interconversion between alcohols and aldehydes or ketones with the reduction of nicotinamide adenine dinucleotide (NAD+ to NADH). In biotransformation, alcohol dehydrogenases are often used for the synthesis of enantiomerically pure stereoisomers of chiral alcohols.
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Papain (from Carica Papaya Latex)
Supplier: Thermo Fisher Scientific
Papain (from Carica Papaya Latex)
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Uricase
Supplier: Thermo Fisher Scientific
Uricase is very important for the determination of uric acid in biological fluids.