Supplier: VWR Chemicals

A zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA.

Supplier: VWR Chemicals

DEPC is used to inactivate RNase enzymes from water and other laboratory utensils. DEPC covalently modifies histidine residues in RNase enzymes. DEPC can be used with PBS or MOPS buffer, but is incompatible with Tris and HEPES.

Supplier: VWR Chemicals

The volumetric and coulometric reagents are buffered systems, stabilised around the ideal value between 5 to 7 ensuring a rapid and stoichiometric course of reaction. Buffering systems which neutralise and stabilise the pH during the reaction are suitable for this purpose.

Supplier: VWR Chemicals

MES, vattenfri ≥99%, high purity suitable for buffer solutions

Supplier: VWR Chemicals

Tris-(hydroximetyl) aminometan ≥99.9% buffer substance, for analysis and biochemical purposes

Supplier: VWR Chemicals

Ready-to-use, single-phase solution that does not have to be equilibrated with a buffering purification solution prior to use. Premixed with Isoamyl Alcohol (25:24:1).

Supplier: VWR Chemicals

peqGOLD Protein Marker I is a mixture of seven purified proteins which are re-dissolved 'ready to use' in loading buffer. The proteins resolve into sharp, tight bands in the range of 14,4 to 116,0 kDa when analysed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250.

Supplier: VWR Chemicals

A convenient pre-mixed solution that is easily diluted for buffer preparation under denaturing conditions.Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions.

Supplier: VWR Chemicals

VWR® dehydrated culture media that has been granulated offers both convenience and safety benefits. The correct dissolution of culture media is critical to achieving the best performance. Granulated culture media saves time in the preparation of ready to use culture media. In addition, granulation reduces dust generation, so handling the product is more pleasant and safer for the user, making it a much cleaner and safer working environment.

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Supplier: VWR Chemicals

Indicator tablets for determination of water hardness by titration with EDTA.

Specifications
Minimum weight: ≥ 0.20 g
Maximum weight: ≤ 0.23 g
Disintegration time (at 25°C): ≤ 150 s
Application test: conforms

The tablets are dissolved in 100 ml of the water sample to be tested, which has been buffered at pH 10 with 1 ml of Ammonia/ Ammonium chloride buffer solution, and then titrated with EDTA solution (0,01 M) till the sample turns from red to blue.
Total hardness in mg/l = 10×ml of titrant used.

Control samples of  calcium carbonate should be used to calibrate the method.

Supplier: VWR Chemicals

Hepes is a widely used buffer in biological studies. In cell culture media, it is employed as a substitute for the bicarbonate buffer at a concentration of 25 mM or as a supplement to the bicarbonate buffer (concentration 10 to 15 mM).

Supplier: VWR Chemicals

Reagent for stabilisation of buffer formulations and electrophoresis of biological molecules.

Supplier: VWR Chemicals

Reagent for stabilisation of buffer formulations and electrophoresis of biological molecules.

Supplier: VWR Chemicals

Reagent for stabilization of buffer formulations and electrophoresis of biological molecules.

Supplier: VWR Chemicals

A range of culture media in compliance with ISO standards and the International Harmonised Pharmacopoeia. All our media are, as you would expect, produced using high quality raw materials and are subject to strict quality controls, in a production environment following Good Manufacturing Practice guidelines. Raw materials are selected in compliance with the required standards. Dehydrated culture media should be stored at 10 to 30 °C unless otherwise stated, in their closed containers in a dry environment. Standard pack size is 500 g.

Supplier: VWR Chemicals

Phenol:Chloroform pH 6.7/8.0 is premixed with isoamyl alcohol (25:24:1). A phenol:chloroform mixture is ideal for the extraction of protein from DNA preparations. Packaged at pH 6.7, this product is accompanied by a separate alkaline buffer which allows the researcher to increase the pH to 8.0. Doubly distilled, high purity phenol packaged under nitrogen. Safer, easier alternative to crystalline phenol.

Supplier: VWR Chemicals

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.

Supplier: VWR Chemicals

(= 10% Formaline solution)

Supplier: VWR Chemicals

Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH. Universally applicable buffers for biochemistry must be water soluble, not interfere with biological processes.

Supplier: VWR Chemicals

Ethidium bromide solution is a fluorescent stain for visualising nucleic acids in solution or in electrophoresis gels. The solution is provided at a concentration of 0,625 mg/ml. A final working concentration of 0,5 g/ml is easily obtained by adding one drop of solution to 50 ml of molten agarose, running buffer or staining buffer. Ethidium bromide solution in dropper bottles may be stored at room temperature for up to one year.

Supplier: VWR Chemicals

Strong detergent utilised in lysis buffers for the complete disruption of membranes and denaturation of proteins.

Supplier: VWR Chemicals

Strong detergent utilised in lysis buffers for the complete disruption of membranes and denaturation of proteins.

Supplier: VWR Chemicals

Biological buffers are useful for cell culture in vitro, enzyme assays, and some electrophoretic applications at physiological pH.

Supplier: VWR Chemicals

Biological buffers are useful for cell culture in vitro, enzyme assays, and some electrophoretic applications at physiological pH.

Supplier: VWR Chemicals

HEPES is a general purpose buffer available for biological research.

Supplier: VWR Chemicals

Reagent for stabilisation of buffer formulations and electrophoresis of biological molecules.

Supplier: VWR Chemicals

A biological buffer used for cell culture, in vitro, enzyme assays and some electrophoretic applications at physiological pH.

Supplier: VWR Chemicals

Nucleic Acid Purification Kits and Reagents, RNA Lysis Buffer T

Supplier: VWR Chemicals

Purifies DNA bands 70 bp to 20 kb in length from agarose gels. The DNA band of interest is excised from the gel, dissolved in binding buffer, and efficiently processed in a peqGOLD DNA column. >90% recovery. Elution volume: 30 to 50 µl.