"protein purification system"

Supplier: G-Biosciences

The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.

Supplier: G-Biosciences

Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.

Supplier: Biotium

Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.

Supplier: Abcam

Anti-HA Affinity Resin - Amintra (ab270603) is designed for simple, rapid HA-tagged recombinant protein purification and immunoprecipitation.

Supplier: G-Biosciences

The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: Avantor

The J.T.Baker^® BAKERBOND^® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.

Supplier: Rockland Immunochemicals

Protein G is a surface protein of two groups of streptococcal bacteria that has ability to bind immunoglobulins and also to albumins. Protein G are prepared from chromatographically pure protein G.

Supplier: Biotium

Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.

Supplier: G-Biosciences

Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

Supplier: Thermo Fisher Scientific

The T7 cell-free expression vectors (pT7CFE1) are cloning plasmids optimised for use with the Thermo Scientific™ 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation.

Supplier: Abcam

His-Tag Protein Purification Column (Pre-packed, 5 x 5ml) - AminTrap (ab270529) is a simple and easy ready to use chromatography medium for purifying His-tagged proteins that have been expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells.

Supplier: Thermo Fisher Scientific

The Thermo Scientific KingFisher Apex represents the pinnacle of automated purification for DNA, RNA, proteins, and cells. Building on decades of KingFisher product value and excellence, we’ve combined superior instrument capabilities with complete touch screen-based control to deliver unrivalled flexibility and performance. The KingFisher Apex makes sample prep simple.

Supplier: G-Biosciences

The Pearl™ IgG purification resin allows for the one-step purification of immunoglobulin G from serum. The resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128747 quickly removes these contaminants.

Supplier: OMNIFIT

EZ columns are designed to suit the majority of standard laboratory chromatography applications. They are ideal for aqueous systems and compatible with solvents used in common liquid chromatography applications such as protein purification.

Supplier: Abcam

GTP-Agarose (ab270533) comprises GTP attached to agarose beads.