610 Resultat för: "chromatography preparative"

Supplier: Cytiva

Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range 5 to 5000 kD.

Supplier: Resonac

Asahipak GF-HQ series are polyvinyl alcohol-based material. Designed for both aqueous and organic solvent systems.

Supplier: VARIAN

The EasiCal system for organic solvents consists of two different combs, each with ten detachable spatulas supporting a mixture of five polymer standards with MWs selected to provide equidistant calibration points for greater accuracy.

Supplier: Cytiva

HiPrep™ 16/60 Sephacryl® S-200 HR and HiPrep™ 26/60 Sephacryl® S-200 HR are pre-packed gel filtration columns for preparative separation of biomolecules of up to 5 and 13 ml sample volume respectively. Sephacryl® S-200 is used for separating mid-size proteins including antibodies and serum proteins.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Pierce™ peptide desalting spin columns are ready to use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. The polymer-based hydrophobic resin contained in each column provides excellent binding and recovery characteristics for peptide samples in preparation for mass spectrometry and other methods.

Supplier: VARIAN

The PLRP-S family of products consists of a range of pore sizes and particle sizes, all with identical chemistry and fundamental adsorptive characteristics. The particles are inherently hydrophobic so there is no bonded phase, alkyl ligand required for reversed-phase separations. This gives a highly reproducible material that is free from silanols and heavy metal ions. Within the extensive product range are columns suitable for analytical separations and preparative purifications.

Supplier: Cytiva

HiPrep™ 16/60 Sephacryl® S-400 HR and HiPrep™ 26/60 Sephacryl® S-400 HR are pre-packed gel filtration columns for preparative separation of biomolecules of up to 5 and 13 ml sample volume respectively. Sephacryl® S-400 is used for separating polysaccarides, macromolecules with extended structures, and even small plasmids.

Supplier: Cytiva

HiPrep™ 16/60 Sephacryl S-500 HR and HiPrep™ 26/60 Sephacryl® S-500 HR are pre-packed gel filtration columns for preparative separation of biomolecules of up to 5 and 13 ml sample volume respectively. Sephacryl® S-500 is used for separating polysaccarides, macromolecules with extended structures, and even small plasmids.

Supplier: Resonac

Polymer-based columns usable in 100% water and buffer solutions. Ideal for the analysis of basic substances.

Supplier: HAMILTON BONADUZ

PRP-h5 polimeric reversed phase columns are ideal for applications that require a high degree of column-to-column and batch to batch consistency (quality assurance, clinical trials). Unlike C8 or C18 columns, PRP-h5 has no stationary phase coating. The integral reversed phase characteristics of the PRP-h5 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyse, the column maintains its performance characteristics longer than many C8 or C18 columns. PRP-h5 columns are packed with a poly(styrene-divinylbenzene) material.

Supplier: Cytiva

Sephadex™ G-50 M DNA Grade chromatography resin for purification of DNA fragments up to 20 bases in length from small molecules such as salts by size exclusion.

Supplier: HAMILTON BONADUZ

A fast, versatile HPLC column packing material. These columns rapidly and easily separate closely related alcohols and organic acids without column heating. The sulfonated poly(styrene-divinylbenzene) support separates samples by a mixed mode mechanism. A wide range of samples can be analysed with these columns because the selectivity of the column can be altered by changing the pH of the buffer or adding an organic modifier (methanol, acetonitrile) to the buffer. The stability to organic solvents makes it possible to analyse samples that have been too highly retained on conventional ion exclusion supports.

Supplier: Cytiva

For rapid purification of labelled DNA (≥20 bases) from unincorporated labelled nucleotides in a volume of 25 to 50 μl using spin column chromatography.

Supplier: Cytiva

Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.

Supplier: Cytiva

illustra™ MicroSpin™ S-400 HR columns designed for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography.

Supplier: Whatman products (Cytiva)

Whatman Puradisc micron syringe filters from Cytiva's offer a versatile solution for analytical sample preparation needs. They are a variety of options available to suit all labs needs, and are well-suited for routine syringe filtration of samples up to 100 mL for a range of applications, including high performance liquid chromatography (HPLC) and capillary electrophoresis (CE).

Supplier: MP Biomedicals

Goat IgG fraction to beta-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques.
The antibody is produced by hyperimmunizing goats with the enzyme beta-galactosidase from Escherichia coli. The IgG fraction is prepared by delipidation, ammonium sulfate fractionation, and anion exchange chromatography, and is further purified by solid-phase absorption to remove antibodies to non-beta-galactosidase E. coli proteins. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed. No preservative is added.

Supplier: VWR Collection

Experience the next level of high-performance liquid chromatography with the Chromaster Plus HPLC system. This latest version boasts a sleek new design and enhanced performance features, including the upgraded CM5310 column oven and CM5410 UV detector. The new design offers a modern and user-friendly interface, while the enhanced performance of the CM5310 column oven and CM5410 UV detector ensures superior results. Additionally, users can enjoy peace of mind with an extended warranty coverage.

Supplier: Cytiva

ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.

Supplier: MP Biomedicals

The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative is added.

The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Hemagglutination titer is standardized with an in-house control by measurement of agglutination end-point with 10% human red blood cells. Each IgG fraction is tested for purity at a minimum of 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected.

Supplier: MERCK PRODUCTION CHEMICALS

Micro-crystalline cellulose is a hydrophilic polysaccharide packing material preferred for the raw separation of amino acids and related compounds. Cellulose is also used for the gentle purification of bio-molecules. Because of the organic nature of cellulose, it can only be used in pre-swollen state and under gentle or hydrostatic pressure.

Supplier: Cytiva

illustra™ MicroSpin™ S-300 HR columns designed for rapid purification of PCR products (>100 bp) from unincorporated primers (<20-mers) and nucleotides using spin-column chromatography.

Supplier: HAMILTON BONADUZ

PRP-1 polymeric HPLC columns are designed for broad ranging uses, similar to C8 or C18 HPLC materials. They combine the best characteristics of silica-based and polymeric columns resulting in highly inert and long-lasting columns. These columns are ideal for applications that require a high degree of column to column and batch to batch consistency (quality assurance, clinical trials). Unlike C8 or C18 columns, PRP-1 has no stationary phase coating. Since there is no stationary phase to hydrolyse, the column maintains its performance characteristics longer than many C8 or C18 columns.

Supplier: HICHROM

Whelk-0®1 is the most widely applicable chiral phase, due to the incorporation of both p-acceptor and p-donor characteristics.

Supplier: Resonac

SB-800 HQ are polyhydroxymethacrylate-based SEC columns for the molecular weight distribution analysis of water-soluble polymers. The solvent can be substituted with DMF (except for SB-802 HQ and SB-807 HQ columns), enabling the analysis of polar polymers.

Supplier: MP Biomedicals

This product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. Specificity for human IgG is determined by immunoelectrophoresis (IEP) versus purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins.Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation in the gamma region.

Supplier: MP Biomedicals

Product is rabbit IgG (whole molecule) covalently coupled to Sepharose-4B, and packed in a polystyrene chromatography column.IgG affinity gel contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because this gel is not a bind-and-release support, it is extremely fast and gentle to antibodies, resulting in antibody preparations of high purity and high activity. IgG affinity gel works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with this gel are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.

Supplier: MP Biomedicals

Sodium phosphate dibasic heptahydrate is a reagent with very high buffering capacity widely used in molecular biology, biochemistry and chromatography. Sodium phosphate dibasic is highly hygroscopic and water soluble. Useful in conjunction with Sodium Phosphate, Monobasic in the preparation of biological buffers.

Supplier: Cytiva

For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.

Supplier: MERCK PRODUCTION CHEMICALS

Består av magnesium och kiseloxider i förhållandet 85:15. Detta är en polär fas som används för kromatografi av exempelvis steroider och alkaloider. Aktiviteten bestäms vid vilken temperatur materialet aktiveras.