buffers
Buffertar skyddar prover mot pH-förändringar när syror eller alkalier tillsätts. Pulver- och flytande former spårar effektivt proteiner under elektroforesisolering och separation. Saltbuffertarna kan också användas för att kalibrera och standardisera pH-mätare för noggranna avläsningar. Blockerande ämnen hindrar antikroppar från att fästa vid membranfria platser som skulle kunna producera en störande bakgrund. Buffertarna finns i olika koncentrationer för att passa alla behov och kan komma i flytande eller dehydrerade format.
Dulbecco's Phosphate Buffered Saline (D-PBS) without Ca++ and Mg++
Supplier: Stemcell Technologies
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.
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ALDEFLUOR™ Assay Buffer
Supplier: Stemcell Technologies
Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.
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HEPES Buffer Solution (1 M)
Supplier: Stemcell Technologies
HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.
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RoboSep™ Buffer 2
Supplier: Stemcell Technologies
RoboSep™ buffer 2 is recommended for select EasySep™ cell separation protocols.
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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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Dulbecco's Phosphate Buffered Saline (D-PBS), 10X Concentrate without Ca++ and Mg++
Supplier: Stemcell Technologies
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.
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Fixation Buffer
Supplier: Stemcell Technologies
Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.
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Intracellular Permeabilisation Buffer (10X)
Supplier: Stemcell Technologies
Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.
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RoboSep™-C PBS/EDTA Buffer
Supplier: Stemcell Technologies
PBS buffer containing EDTA.
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