142 Resultat för: "TriLink BioTechnologies"

Supplier: TriLink BioTechnologies

Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.

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This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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The Antibody-Oligonucleotide All-in-One Conjugation kit offers TurboLink™ catalysed conjugation based on Solulink’s proprietary linking technology to prepare antibody-oligonucleotide conjugates without chromatography. The kit includes buffers, spin columns, and a calculator to determine MSR. No column chromatography is required. With the Antibody-Oligonucleotide All-in-One Conjugation kit, you can generate high-purity conjugates virtually free of residual antibody or oligonucleotide (>95% conjugate).

Supplier: TriLink BioTechnologies

Fluorescein Antibody Conjugation kit offers an efficient method that delivers >95% conversion of antibody to conjugate. The kit includes everything needed to make two direct-labelled fluorescein-antibody conjugates from 100 µg of any user-supplied antibody.

Supplier: TriLink BioTechnologies

Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest, and the Cas9 protein is employed to perform double-stranded DNA cleavage.

Supplier: TriLink BioTechnologies

Biotin-16-Aminoallyl-2'-deoxyuridine can be enzymatically incorporated into DNA via PCR, nick translation, primer extension using Klenow(exo-) DNA polymerase, and terminal deoxynucleotidyl transferase (TdT). Biotinylated cDNA can be prepared with this NTP using Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.

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TriLink® N1-Methyl-2'-O-Methylpseudouridine-5'-Triphosphate

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TriLink® Cytidine-5'-O-(1-Thiotriphosphate)

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This construct expresses a firefly luciferase (FLuc) protein, originally isolated from the firefly Photinus pyralis, as self-amplifying RNA (saRNA). FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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Protein assay, Conjugation reagent, Sulfo-S-4FB Crosslinker (water soluble)

Supplier: TriLink BioTechnologies

This deoxyguanosine analog lacks the N7 nitrogen found in the natural guanine nucleobase. Replacement of this nitrogen atom with a carbon atom results in diminished structure of the major groove in DNA and may disrupt base stacking, but does not interfere with Watson-Crick base pairing. This is desirable for applications such as amplification and sequencing of GC-rich nucleic acid sequences.

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EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA in vivo resulted in significant increases of both reticulocyte counts and hematocrits.

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TriLink® 2'-Deoxythymidine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

2',3'-Dideoxyguanosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddGTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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2',3'-Dideoxythymidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddTTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

Supplier: TriLink BioTechnologies

N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.).

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TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine

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The R-PE-Antibody Conjugation kit offers an innovative catalysed conjugation that delivers >95% conversion of antibody to conjugate. The kit includes everything needed to make two direct-labelled R-PE antibody conjugates from 500 µg of any user-supplied antibody.

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ChromaLink™ Didoxigenin contains a UV-traceable chromophore to enable reproducibility labelling. The degree of biotinylation can be measured in minutes, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan, biotin incorporation can be quantified and reproducible production of consistent batches ensured. ChromaLink™ labelling can be paired with NanoLink™ Streptavidin Magnetic Beads for many types of assay development.

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The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

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Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

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2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.

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TriLink® 5-Propargylamino-2'-deoxycytidine-5'-Triphosphate

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TriLink® 2'-O-Methylcytidine-5'-Triphosphate

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2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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N,N-Dimetylformamid, vattenfri

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TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

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TriLink® N1-Propylpseudouridine-5'-Triphosphate

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β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

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TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate