29 Resultat för: "SGI - DNA Synthetic Genomics"

Supplier: SGI - DNA Synthetic Genomics

The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

Supplier: SGI - DNA Synthetic Genomics

The Gibson Assembly® Method is a well-established assembly reaction that can be leveraged to join multiple, mutagenised DNA fragments with overlapping ends. Following mutagenesis, DNA fragments of various lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach lends itself to small and large constructs and encompasses both single and multiple insert assemblies.

Supplier: SGI - DNA Synthetic Genomics

Gibson Assembly® Positive Control, 5 reactions

Supplier: SGI - DNA Synthetic Genomics

Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Supplier: SGI - DNA Synthetic Genomics

The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

Supplier: SGI - DNA Synthetic Genomics

The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

Supplier: SGI - DNA Synthetic Genomics

Chemically competent cells, Vmax™ Express

Supplier: SGI - DNA Synthetic Genomics

The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

Supplier: SGI - DNA Synthetic Genomics

[EN]VMAX EXPRESS ELECTROCOMPETENT CELLS 1 * 20 Vial

Supplier: SGI - DNA Synthetic Genomics

VMAX X2 CHEMICALLY COMPETENT CELLS 20R 1 * 20 Vial

Supplier: SGI - DNA Synthetic Genomics

VMAX EXPRESS CHEMICALLY COMPETENT CELLS 1 * 5 Vial

Supplier: SGI - DNA Synthetic Genomics

[EN]VMAX EXPRESS ELECTROCOMPETENT CELLS 1 * 1.000 ml

Supplier: SGI - DNA Synthetic Genomics

[EN]BIOXP MODULE A16-DNA LIBRARY 1 * 1 Items

Supplier: SGI - DNA Synthetic Genomics

[EN]BIOXP MODULE B16-DNA ASSEMBLY 1 * 1 Items

Supplier: SGI - DNA Synthetic Genomics

[EN]BIOXP MODULE B32 - DNA ASSEMBLY 1 * 1 Items

Supplier: SGI - DNA Synthetic Genomics

[EN]KIT GIBSON ASSEMBLY HIFI STARTER 50% 1 * 5 Tests

Supplier: SGI - DNA Synthetic Genomics

[EN]VMAX EXPRESS ELECTROCOMPETENT CELLS 1 * 10 Items

Supplier: SGI - DNA Synthetic Genomics

[EN]GIBSON ASSEMBLY ULTRA KIT STARTER 50 1 * 5 Tests

Supplier: SGI - DNA Synthetic Genomics

[EN]BIOXP 9600 SYSTEM 1 * 1 Items

Supplier: SGI - DNA Synthetic Genomics

[EN]BIOXP 3250 KIT N-SCRIPT TEST PANEL 1 * 1 Items

Supplier: SGI - DNA Synthetic Genomics

[EN]KIT GIBSON ASSEMBLY HIFI HC 1STEP 10 1 * 10 Tests

Supplier: SGI - DNA Synthetic Genomics

[EN]BIOXP MODULE A32- DNA LIBRARY - 32 S 1 * 1 KIT

Supplier: SGI - DNA Synthetic Genomics

[EN]MASTER MIX GIBSON ASSMBLY HIFI HC 10 1 * 10 Tests

Supplier: SGI - DNA Synthetic Genomics

[EN]VMAX EXPRESS CHEMICALLY COMPETENT C 1 * 10 Vial

Supplier: SGI - DNA Synthetic Genomics

[EN]BIOXP SYSTEM TIP 1 SET (3.5L RACK 1 1 * 1 SET

Supplier: SGI - DNA Synthetic Genomics

KIT GIBSON ASSEMBLY SDM 1 * 10 Tests

Supplier: SGI - DNA Synthetic Genomics

[EN]GIBSON ASSEMBLY SDM KIT STARTER 50 % 1 * 5 Tests

Supplier: SGI - DNA Synthetic Genomics

[EN]BIOXP 3250 PREVENTATIVE MAINTENANCE 1 * 1 Items

Supplier: SGI - DNA Synthetic Genomics

[EN]BIOXP 9600 SYSTEM 1 * 1 Items