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qPCR cycler, Q

qPCR cycler, Q

Supplier: Quantabio

Q qPCR cycler uses a patented magnetic induction technology to rapidly heat samples, coupled with fan-forced air for cooling to acquire data in only 25 minutes. Available as four channel models, the robust optical system acquires all channels simultaneously and allows for running the fastest multiplexed assays.

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cDNA-syntes, qScript™

cDNA-syntes, qScript™

Supplier: Quantabio

qScript™ cDNA-synteskit innehåller qScript™, reverstranskriptas, en blandning av MMLV, reverstranskriptas, och ribonukleashämmande protein, som optimerats för känslig och tillförlitlig cDNA-syntes med ett brett dynamiskt omfång av införd RNA. cDNA-syntesreagens finns i flera olika supermixar och kit som passar specifika tillämpningar.

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AccuStart™ II PCR Genotyping Kit

AccuStart™ II PCR Genotyping Kit

Supplier: Quantabio

The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

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AccuStart™ II GelTrack PCR SuperMix

Supplier: Quantabio

AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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Virus detection kit, qScript™ 1-Step Virus ToughMix®

Virus detection kit, qScript™ 1-Step Virus ToughMix®

Supplier: Quantabio

Superior sensitivity for viral RNA detection.

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repliQa HiFi ToughMix

repliQa HiFi ToughMix

Supplier: Quantabio

Superior speed and inhibitor tolerance.

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sparQ mRNA-Seq Kit

sparQ mRNA-Seq Kit

Supplier: Quantabio

Ultra-sensitive mRNA library prep workflow with efficient poly(A) capture.

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PerfeCTa® qPCR FastMix™-reaktionsblandningar

Supplier: Quantabio

FastMixes innehåller buffertar och stabiliseringsmedel som optimerats för högsta PCR-effektivitet, reproducerbarhet och känslighet tillsammans med TaqMan® och TaqMan® MGB-prober. En nyckelkomponent i PerfeCTa® qPCR FastMixes är AccuFast™ Taq DNA-polymeras som möjliggör omedelbar aktivering och snabb polymerisationskinetik. Vid värmeinaktivering (20 sekunder vid 95 °C) denatureras antikropparna irreversibelt och frigör fullt aktivt, omodifierat Taq DNA-polymeras. Detta underlättar specifik och effektiv primerextension i rumstemperatur. FastMixes ger maximal PCR-effektivitet, -känslighet och -specificitet och robust fluorescerande signal med antingen snabbt eller konventionellt cyklingsprotokoll.

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PCR Master Mix, sparQ HiFi

PCR Master Mix, sparQ HiFi

Supplier: Quantabio

sparQ HiFi PCR Master Mix was specially designed for high efficiency library amplification from low DNA input. sparQ HiFi PCR Master Mix minimises the number of amplification cycles required to achieve the threshold required for sequencing. The result is a superior performing high fidelity master mix that increases library yield which reduces PCR-derived artifacts for a variety of sequencing applications.

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eQo One-Step ToughMix®

eQo One-Step ToughMix®

Supplier: Quantabio

No compromises. Unparalleled performance meets sustainability.

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Genomic DNA extraction from dried blood spots, Extracta™ DBS

Genomic DNA extraction from dried blood spots, Extracta™ DBS

Supplier: Quantabio

Extracta™ DBS is a ready to use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman® 903 filter/TFN or 226 from Ahlstrom Munksjö paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix® or PerfeCTa MultiPlex qPCR ToughMix® enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

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qPCR SuperMixes, PerfeCTa®

qPCR SuperMixes, PerfeCTa®

Supplier: Quantabio

PerfeCTa® qPCR SuperMixes i 2X koncentration är reaktionsblandningar som är färdiga att använda och som innehåller alla komponenter förutom primrar, prob(er) och templat för qPCR.

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DNA fragment and library prep kit, sparQ

DNA fragment and library prep kit, sparQ

Supplier: Quantabio

The sparQ DNA Frag and Library Prep kit optimises the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimising DNA over fragmentation while greatly simplifying library.

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AccuStart™ II Taq DNA polymerase

Supplier: Quantabio

AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

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DNA prep for PCR - tissue, Extracta™

Supplier: Quantabio

The Extracta™ DNA prep for PCR - tissue consists of a two component reagent kit for rapid and efficient extraction of PCR-ready genomic DNA from mammalian tissues. The kit has been demonstrated to work with mouse tail biopsies, ear punches and a variety of human tissue samples including hair, buccal cells and saliva.

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AccuStart™ Genotyping ToughMix®

AccuStart™ Genotyping ToughMix®

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

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