BspQI

 BspQI (isoschizomers: SapI, PciSI and LguI) is a Type IIS restriction enzyme known for its unique cleavage properties. It recognizes asymmetric DNA sequences, cleaves the double-stranded DNA outside of the recognition site, and generates a 5′ overhang.

• BspQI’s recognition and cleavage sites:

• 5' GCTCTTCN↓ 3'
• 3' CGAGAAGNNNN↑ 5'

BspQI is thermostable and can be used in various molecular biology applications, including generation of linear DNA templates for in vitro transcription to synthesize RNA.

The enzyme is provided 10 U/μl in storage buffer. It is also supplied with 1.25 ml of 10X RE Reaction Buffer (500 mM Tris,1000 mM NaCl, 100 mM MgCl2, 1 mg/ml rHSA, pH 7.5 at 25 °C).

Unit definition: The amount of BspQI required to digest 1 µg of lambda DNA in 1 hour at 50 °C in a 50 µl reaction.

Concentration: 10 U/µl