About this item
Pellet Paint® Co-Precipitant är en synlig, infärgad carriermolekyl speciellt utarbetad för användning vid alkoholutfällning av nukleinsyror. Reaktionen tar 2 minuter och kräver ej inkubation vid låg temperatur eller förlängd centrifugering. Både RNA och DNA utfälls effektivt, även i de mest utspädda lösningar (2 ng/ml) och pelleten med dess rosa färg spåras enkelt. Pelleten kan enkelt följas under tvättstegen, vilket förhindrar förluster under hantering och säkerställer att pelleten resuspenderas.
- Suitable for the rapid, quantitative precipitation of DNA and RNA, including PCR clean-up and template preparation
- Compatible with most molecular biology procedures and free of contaminating nucleic acids and nucleolytic enzymes
- Although Pellet Paint absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible using the absorbance ratio provided as a correction factor when determining nucleic acid concentration
- Compatible with Cy5®-based automated sequencers
Most PCR applications benefit from a clean-up step in which primers and other reactants are removed and the target DNA is concentrated. Pellet Paint Co-Precipitant is ideal for this purpose because the procedure is rapid, primers <50 nt in length are efficiently removed, and the DNA is quantitatively recovered. Furthermore, it is easy to tell when the DNA has been completely re-suspended following the precipitation step.
Specifications
- Method / Format:Precipitation
- Prep size:Scalable
- Refine target molecule:Total RNATotal DNAAmplified/modified DNA
- Sample type:RNA prepsDNA prepsPCR and other enzymatic reactions
- Target molecule:DNARNA
- Benämning:Pellet Paint® Co-Precipitant