Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.

• For affinity chromatography
• Fast, one-step purification of GST-tagged proteins
• Mild elution conditions preserving protein antigenicity and function
• For scale-up due to high flow properties
• Glutathione coupled to Sepharose 4 Fast Flow via a 10-carbon spacer arm
• Epoxy activation coupling method
• Stable in all commonly used aqueous buffers

Mild elution conditions preserve protein antigenicity and function. Sepharose 4 Fast Flow provides good flow properties and is excellent for scaling up. Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.