For digestion of DNA.

• Effectively eliminate DNA contaminants from dissociation medium

Use Deoxyribonuclease I (DNase I) for routine tissue dissociation, to minimise cell clumping, and eliminate DNA contaminants from dissociation medium. This endonuclease consists of a single glycosylated polypeptide chain with two disulfide bonds. It preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for DNA digestion in human cells and tissues such as microglia (Klegeris and McGeer), cartilage (Dunham and Koch), colon (Fukushima and Fiocchi), epithelium (Fukushima and Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural cells (Fuja et al.), and stem cells (Kusuma et al.).