Human alpha MSH ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human alpha MSH in serum, plasma, tissue homogenates, and other biological fluids.

• Ready-To-Use ELISA Kit
• Detection Range: 12,5 - 800 pg/ml
• Sensitivity: 7,5 pg/ml
• Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%

Human alpha MSH ELISA kit (A78988) employs the competitive enzyme immunoassay technique for the quantitative measurement of human alpha MSH in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with alpha MSH antigen. During the incubation, alpha MSH present in the samples or standards competes with the fixed amount of immobilized alpha MSH for binding sites on the Biotinylated Anti-alpha MSH Antibody. The more alpha MSH present in a sample or standard, the less Biotinylated Anti-alpha MSH Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-alpha MSH Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of alpha MSH present in each sample or standard. The concentration of alpha MSH can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.