Mouse Luteinizing Hormone ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse Luteinizing Hormone in serum, plasma, tissue homogenates, and other biological fluids.

• Ready To Use ELISA Kit
• Detection Range: 0,469 to 30 ng/ml
• Sensitivity: 0,281 ng/ml
• Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Mouse Luteinizing Hormone ELISA Kit (A79500) employs the competitive enzyme immunoassay technique for the quantitative measurement of mouse Luteinizing Hormone in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Luteinizing Hormone antigen. During the incubation, Luteinizing Hormone present in the samples or standards competes with the fixed amount of immobilized Luteinizing Hormone for binding sites on the Biotinylated Anti-Luteinizing Hormone Antibody. The more Luteinizing Hormone present in a sample or standard, the less Biotinylated Anti-Luteinizing Hormone Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Luteinizing Hormone Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Luteinizing Hormone present in each sample or standard. The concentration of Luteinizing Hormone can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.