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Human Hormone Sensitive Lipase/HSL ELISA Kit
  ANTIA79037-96
New Product
 :  
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Human Hormone Sensitive Lipase/HSL ELISA Kit
  ANTIA79037-96
New Product
 :  

 

  • Förp.:
    96 Tests
  • Analysens varaktighet:
    Multiple steps
  • Analystyp:
    Sandwich
  • Formatera:
    Pre-coated
  • Primary antibody reactivity:
    Human
  • Target protein:
    Hormone Sensitive Lipase/HSL
  • Beskrivning:
    Human Hormone Sensitive Lipase/HSL ELISA kit
  • Typ av prov:
    Serum, plasma, tissue homogenates and other biological fluids
  • Korsreaktivitet:
    Human Hormone Sensitive Lipase/HSL ELISA Kit exhibits high specificity and excellent specificity for the detection of human Hormone Sensitive Lipase/HSL. No significant cross-reactivity or interference between Hormone Sensitive Lipase/HSL and analogues was observed.
  • Detektionsmetod:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Analysprincip:
    Quantitative
  • Hållbarhetstid:
    Store for 6 months at 4 °C
  • Detektionsområde:
    31,25 - 2000 pg/ml
  • Förvaringstemperatur:
    4 °C
  • Provvolym:
    100 μl
  • Känslighet:
    18,75 pg/ml
  • Regulatory status:
    RUO

 

 

Human Hormone Sensitive Lipase/HSL ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Hormone Sensitive Lipase/HSL in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready To Use ELISA Kit
  • Detection Range: 31,25 to 2000 pg/ml
  • Sensitivity: 18,75 pg/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Human Hormone Sensitive Lipase / HSL ELISA Kit (A79037) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Hormone Sensitive Lipase / HSL in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Hormone Sensitive Lipase / HSL has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Hormone Sensitive Lipase / HSL present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Hormone Sensitive Lipase / HSL Antibody, which binds the captured Hormone Sensitive Lipase / HSL present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Hormone Sensitive Lipase / HSL captured in each well. The concentration of Hormone Sensitive Lipase / HSL can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.