Serum- and xeno-free medium and attachment substrate for expansion of human pluripotent stem cell-derived endothelial cells.

Efficiently expand human pluripotent stem cell (hPSC)-derived endothelial cells using xeno-free STEMdiff™ Endothelial Expansion Culture Kit (Catalog #100-1218). This kit includes xeno-free STEMdiff™ Endothelial Expansion Basal Medium and 5X Supplement, and Animal Component-Free Cell Attachment Substrate, and is is optimised for the expansion of hPSC-derived endothelial cells and for the maintenance of endothelial marker expression. After 7 days of mesoderm and endothelial induction, the resulting cells can be expanded for 5 - 6 passages to generate large numbers of highly pure, mature endothelial cells that typically reach ≥ 99% CD144+CD31+ by passage 2 and maintain this phenotype until passage 5. This kit is compatible with hPSCs maintained in mTeSR™1, mTeSR™ Plus, or TeSR™-E8™ on Corning® Matrigel®. The components of STEMdiff™ Endothelial Expansion Culture Kit are included in STEMdiff™ Endothelial Differentiation Kit. For passaging, Animal Component-Free Cell Dissociation Kit is required.

NOTE: Heparin Solution is required for preparation of complete STEMdiff™ Endothelial Expansion Medium. CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate. This product may be used for research purposes only. Please contact STEMCELL for any clinical or commercial application of the Product.