Defined, xeno-free induction medium for early mesodermal differentiation.

STEMdiff™ Mesoderm Induction Medium (MIM) is a defined, xeno-free medium for generation of early mesoderm cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Protocols for mesodermal differentiation can be difficult and inconsistent, therefore, use the short and simple STEMdiff™ MIM monolayer protocol to differentiate your human pluripotent stem cells (hPSCs). STEMdiff™ MIM is a complete medium that produces a cell population enriched for early mesoderm, as indicated by positive expression of Brachyury (T) and NCAM markers. As part of the hPSC workflow, STEMdiff™ MIM efficiently differentiates hPSCs cultured in TeSR™ media. When directed, early mesoderm cells produced using STEMdiff™ MIM can be further differentiated to specialised cell types, such as osteoblasts, chondrocytes, adipocytes or endothelial cells.