Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.

• For 5 lab groups
• Complete in 60 minutes

Also required: Water bath, 95 to 100% isopropanol. For optional electrophoresis: Electrophoresis tank, power supply, 5 to 50 µl adjustable or 40 µl fixed volume micropipettes, and white light box.