Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

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Behövs också: elektroforesutrustning, strömkälla, mikropipetter (5–50 µl justerbara eller 40 µl fast volym) med spetsar, en ljuslåda med vitt ljus rekommenderas.