Dye tyramide, CF®
Om denna produkt
Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).
- High-density labeling of a target protein or nucleic acid for enhanced immunoflorence sensitivity
- Especially suited for the detection of low abundance target
- Detection sensitivity of over 100-fold compared to conventional procedures
- Enables multiplex multicolor detection, not limited by antibodies from the same host species
- Bright, photostable and water-soluble CF® dyes are excellent options for fluorescent labeling
TSA is a highly sensitive method for differential gene or protein analysis or detection of low-abundance targets, in fluorescent ICC, IHC, and FISH applications. An antibody- or streptavidin-HRP conjugate catalyzes the deposition of fluorescent dye/biotin tyramides on tyrosine residues on and adjacent to a target protein or nucleic acid sequence in situ This results in high-density labeling of the target and significantly improves the detection sensitivity up to 100-fold compared to conventional methods. TSA is particularly advantageous for fluorescence detection in human tissue, where conventional ICC or FISH often fails to provide adequate signal over autofluorescence background. In applications where increased sensitivity is not required, TSA enables the use of significantly lower antibody or probe concentrations for the same level of detection sensitivity thereby reducing issues of non-specific binding or cross-reactivity. Furthermore, since binding of the tyramide label is covalent, a large number of targets can be detected in the same sample using multiple rounds of sequential TSA, in which the availability of antibodies from different host species is not a limitation. TSA also can be easily integrated with conventional immunostaining.
These conjugates are available with Biotium's next-generation CF® dyes, a series of highly water-soluble fluorescent dyes spanning the visible and near-infrared spectrum for labeling biomolecules such as proteins and nucleic acids. Developed in house by Biotium's industry-leading chemists, CF® dyes offer superior brightness, photostability, and signal-to-noise when compared to other commercially available fluorescent dyes.
17 Alternativ tillgängliga nedan
Product Details & Documents
Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).
- High-density labeling of a target protein or nucleic acid for enhanced immunoflorence sensitivity
- Especially suited for the detection of low abundance target
- Detection sensitivity of over 100-fold compared to conventional procedures
- Enables multiplex multicolor detection, not limited by antibodies from the same host species
- Bright, photostable and water-soluble CF® dyes are excellent options for fluorescent labeling
TSA is a highly sensitive method for differential gene or protein analysis or detection of low-abundance targets, in fluorescent ICC, IHC, and FISH applications. An antibody- or streptavidin-HRP conjugate catalyzes the deposition of fluorescent dye/biotin tyramides on tyrosine residues on and adjacent to a target protein or nucleic acid sequence in situ This results in high-density labeling of the target and significantly improves the detection sensitivity up to 100-fold compared to conventional methods. TSA is particularly advantageous for fluorescence detection in human tissue, where conventional ICC or FISH often fails to provide adequate signal over autofluorescence background. In applications where increased sensitivity is not required, TSA enables the use of significantly lower antibody or probe concentrations for the same level of detection sensitivity thereby reducing issues of non-specific binding or cross-reactivity. Furthermore, since binding of the tyramide label is covalent, a large number of targets can be detected in the same sample using multiple rounds of sequential TSA, in which the availability of antibodies from different host species is not a limitation. TSA also can be easily integrated with conventional immunostaining.
These conjugates are available with Biotium's next-generation CF® dyes, a series of highly water-soluble fluorescent dyes spanning the visible and near-infrared spectrum for labeling biomolecules such as proteins and nucleic acids. Developed in house by Biotium's industry-leading chemists, CF® dyes offer superior brightness, photostability, and signal-to-noise when compared to other commercially available fluorescent dyes.
Product Family Options
Produktinformation
- Ex/EmFörp.TillgänglighetPrice
- 92170.CF® 350 tyramideCF® 350 tyramide347/448 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 675,00 kr
- 92198.CF®405L tyramideCF®405L tyramide395/545 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 850,00 kr
- 96057.CF®405M tyramideCF®405M tyramide408/452 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 750,00 kr
- 92197.CF®405S tyramideCF®405S tyramide404/431 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 850,00 kr
- 92171.CF®488A tyramideCF®488A tyramide490/515 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 675,00 kr
- 92199.CF®514 tyramideCF®514 tyramide516/548 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 850,00 kr
- 96066.CF®532 tyramideCF®532 tyramide527/558 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 600,00 kr
- 92172.CF®543 tyramideCF®543 tyramide541/560 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 675,00 kr
- 96077.CF®550R tyramide
- 96021.CF®555 tyramideCF®555 tyramide555/565 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 850,00 kr
- 92173.CF®568 tyramideCF®568 tyramide562/583 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 675,00 kr
- 92174.CF®594 tyramideCF®594 tyramide593/614 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 675,00 kr
- 92194.CF®620R tyramideCF®620R tyramide617/639 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 850,00 kr
- 92175.CF®640R tyramideCF®640R tyramide642/662 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 675,00 kr
- 96022.CF®647 tyramideCF®647 tyramide650/665 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 850,00 kr
- 92195.CF®660R tyramideCF®660R tyramide663/682 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg5 850,00 kr
- 92196.CF®680R tyramideCF®680R tyramide680/701 nm0,5 mgLog in för att kolla tillgänglighet och ert pris1 * 0,5 mg6 125,00 kr
#: 20864.320
Leverantör: VWR Chemicals
#: 83640.320
Leverantör: VWR Chemicals
#: 1.00030.2500
Leverantör: Merck
#: 31770.294
Leverantör: VWR Chemicals
#: 83645.320
Leverantör: VWR Chemicals
#: 1.09439.1000
Leverantör: Merck